Astaxanthin from Shrimp Cephalothorax Stimulates the Immune Response by Enhancing IFN-γ, IL-10, and IL-2 Secretion in Splenocytes of Helicobacter Pylori-Infected Mice
Abstract
:1. Introduction
2. Results
2.1. Astaxanthin Hydrolysis
2.2. Effect of AXT on IFN-γ Production in Splenocytes of H. pylori-Inoculated Mice
2.3. AXT Influences IL-10 Production in Splenocytes of H. pylori-Inoculated Mice
2.4. Effect of AXT on IL-2 Release in Splenocytes of H. pylori-Inoculated Mice
3. Discussion
4. Materials and Methods
4.1. Mice and Housing
4.2. Helicobacter pylori Strain
4.3. Infection of Mice
4.4. Preparation of Astaxanthin in the Lipid Extract
4.5. Detection of Astaxanthin HPLC
4.6. Treatment with Astaxanthin
4.7. Isolation of Splenocytes
4.8. Cytokine Release Assay
4.9. Statistical Analysis
Author Contributions
Funding
Conflicts of Interest
References
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Davinelli, S.; Melvang, H.M.; Andersen, L.P.; Scapagnini, G.; Nielsen, M.E. Astaxanthin from Shrimp Cephalothorax Stimulates the Immune Response by Enhancing IFN-γ, IL-10, and IL-2 Secretion in Splenocytes of Helicobacter Pylori-Infected Mice. Mar. Drugs 2019, 17, 382. https://doi.org/10.3390/md17070382
Davinelli S, Melvang HM, Andersen LP, Scapagnini G, Nielsen ME. Astaxanthin from Shrimp Cephalothorax Stimulates the Immune Response by Enhancing IFN-γ, IL-10, and IL-2 Secretion in Splenocytes of Helicobacter Pylori-Infected Mice. Marine Drugs. 2019; 17(7):382. https://doi.org/10.3390/md17070382
Chicago/Turabian StyleDavinelli, Sergio, Heidi Mikkelsen Melvang, Leif Percival Andersen, Giovanni Scapagnini, and Michael Engelbrecht Nielsen. 2019. "Astaxanthin from Shrimp Cephalothorax Stimulates the Immune Response by Enhancing IFN-γ, IL-10, and IL-2 Secretion in Splenocytes of Helicobacter Pylori-Infected Mice" Marine Drugs 17, no. 7: 382. https://doi.org/10.3390/md17070382
APA StyleDavinelli, S., Melvang, H. M., Andersen, L. P., Scapagnini, G., & Nielsen, M. E. (2019). Astaxanthin from Shrimp Cephalothorax Stimulates the Immune Response by Enhancing IFN-γ, IL-10, and IL-2 Secretion in Splenocytes of Helicobacter Pylori-Infected Mice. Marine Drugs, 17(7), 382. https://doi.org/10.3390/md17070382