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Open AccessArticle

Rhamnose Binding Protein as an Anti-Bacterial Agent—Targeting Biofilm of Pseudomonas aeruginosa

Institute of Molecular and Cellular Biology, National Tsing Hua University, Hsinchu 30013, Taiwan
Simpson Biotech Co., Ltd., Taoyuan 333, Taiwan
Department of Biology, Johns Hopkins University, Baltimore, ML 21218, USA
Department of Pharmaceutical Chemistry, University of Debrecen, Debrecen 4032, Hungary [email protected] (F.D.)
Department of Life Science, National Tsing Hua University, Hsinchu 30013, Taiwan
Molecular Infectious Disease Research Center, Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Taoyuan 333, Taiwan
Author to whom correspondence should be addressed.
Mar. Drugs 2019, 17(6), 355;
Received: 24 May 2019 / Revised: 11 June 2019 / Accepted: 12 June 2019 / Published: 14 June 2019
(This article belongs to the Special Issue Biofilm Inhibitors of Marine Origin)
More than 80% of infectious bacteria form biofilm, which is a bacterial cell community surrounded by secreted polysaccharides, proteins and glycolipids. Such bacterial superstructure increases resistance to antimicrobials and host defenses. Thus, to control these biofilm-forming pathogenic bacteria requires antimicrobial agents with novel mechanisms or properties. Pseudomonas aeruginosa, a Gram-negative opportunistic nosocomial pathogen, is a model strain to study biofilm development and correlation between biofilm formation and infection. In this study, a recombinant hemolymph plasma lectin (rHPLOE) cloned from Taiwanese Tachypleus tridentatus was expressed in an Escherichia coli system. This rHPLOE was shown to have the following properties: (1) Binding to P. aeruginosa PA14 biofilm through a unique molecular interaction with rhamnose-containing moieties on bacteria, leading to reduction of extracellular di-rhamnolipid (a biofilm regulator); (2) decreasing downstream quorum sensing factors, and inhibiting biofilm formation; (3) dispersing the mature biofilm of P. aeruginosa PA14 to improve the efficacies of antibiotics; (4) reducing P. aeruginosa PA14 cytotoxicity to human lung epithelial cells in vitro and (5) inhibiting P. aeruginosa PA14 infection of zebrafish embryos in vivo. Taken together, rHPLOE serves as an anti-biofilm agent with a novel mechanism of recognizing rhamnose moieties in lipopolysaccharides, di-rhamnolipid and structural polysaccharides (Psl) in biofilms. Thus rHPLOE links glycan-recognition to novel anti-biofilm strategies against pathogenic bacteria. View Full-Text
Keywords: rhamnose-binding protein; anti-biofilm; quorum sensing factor; anti-infection rhamnose-binding protein; anti-biofilm; quorum sensing factor; anti-infection
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Fu, T.-K.; Ng, S.-K.; Chen, Y.-E.; Lee, Y.-C.; Demeter, F.; Herczeg, M.; Borbás, A.; Chiu, C.-H.; Lan, C.-Y.; Chen, C.-L.; Chang, M. .-T. Rhamnose Binding Protein as an Anti-Bacterial Agent—Targeting Biofilm of Pseudomonas aeruginosa. Mar. Drugs 2019, 17, 355.

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