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Open AccessArticle

Characterization of an Alkaline Alginate Lyase with pH-Stable and Thermo-Tolerance Property

1
Department of Pharmacology, College of Basic Medicine, Qingdao University, Qingdao 266071, China
2
Department of Rehabilitation Medicine, Qingdao University, Qingdao 266071, China
3
Central Laboratory of Medicine, Qingdao University, Qingdao 266071, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this paper.
Mar. Drugs 2019, 17(5), 308; https://doi.org/10.3390/md17050308
Received: 23 April 2019 / Revised: 11 May 2019 / Accepted: 14 May 2019 / Published: 24 May 2019
Alginate oligosaccharides (AOS) show versatile bioactivities. Although various alginate lyases have been characterized, enzymes with special characteristics are still rare. In this study, a polysaccharide lyase family 7 (PL7) alginate lyase-encoding gene, aly08, was cloned from the marine bacterium Vibrio sp. SY01 and expressed in Escherichia coli. The purified alginate lyase Aly08, with a molecular weight of 35 kDa, showed a specific activity of 841 U/mg at its optimal pH (pH 8.35) and temperature (45 °C). Aly08 showed good pH-stability, as it remained more than 80% of its initial activity in a wide pH range (4.0–10.0). Aly08 was also a thermo-tolerant enzyme that recovered 70.8% of its initial activity following heat shock treatment for 5 min. This study also demonstrated that Aly08 is a polyG-preferred enzyme. Furthermore, Aly08 degraded alginates into disaccharides and trisaccharides in an endo-manner. Its thermo-tolerance and pH-stable properties make Aly08 a good candidate for further applications. View Full-Text
Keywords: Alginate lyase; Thermo-tolerant; pH-stability; Endo-manner; Vibrio sp. SY01 Alginate lyase; Thermo-tolerant; pH-stability; Endo-manner; Vibrio sp. SY01
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Wang, Y.; Chen, X.; Bi, X.; Ren, Y.; Han, Q.; Zhou, Y.; Han, Y.; Yao, R.; Li, S. Characterization of an Alkaline Alginate Lyase with pH-Stable and Thermo-Tolerance Property. Mar. Drugs 2019, 17, 308.

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