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Mar. Drugs 2019, 17(3), 185; https://doi.org/10.3390/md17030185

Expression, Purification and Characterization of Chondroitinase AC II from Marine Bacterium Arthrobacter sp. CS01

1
Laboratory of Applied Microbiology, College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China
2
Shandong Provincial Key Laboratory of Biochemical Engineering, College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Received: 15 February 2019 / Revised: 12 March 2019 / Accepted: 15 March 2019 / Published: 20 March 2019
(This article belongs to the Special Issue Marine Bacteria as Sources of Bioactive Compounds)
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Abstract

Chondroitinase (ChSase), a type of glycosaminoglycan (GAG) lyase, can degrade chondroitin sulfate (CS) to unsaturate oligosaccharides, with various functional activities. In this study, ChSase AC II from a newly isolated marine bacterium Arthrobacter sp. CS01 was cloned, expressed in Pichia pastoris X33, purified, and characterized. ChSase AC II, with a molecular weight of approximately 100 kDa and a specific activity of 18.7 U/mg, showed the highest activity at 37 °C and pH 6.5 and maintained stability at a broad range of pH (5–7.5) and temperature (below 35 °C). The enzyme activity was increased in the presence of Mn2+ and was strongly inhibited by Hg2+. Moreover, the kinetic parameters of ChSase AC II against CS-A, CS-C, and HA were determined. TLC and ESI-MS analysis of the degradation products indicated that ChSase AC II displayed an exolytic action mode and completely hydrolyzed three substrates into oligosaccharides with low degrees of polymerization (DPs). All these features make ChSase AC II a promising candidate for the full use of GAG to produce oligosaccharides. View Full-Text
Keywords: chondroitinase; eukaryotic expression; enzymatic properties; oligosaccharides chondroitinase; eukaryotic expression; enzymatic properties; oligosaccharides
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Fang, Y.; Yang, S.; Fu, X.; Xie, W.; Li, L.; Liu, Z.; Mou, H.; Zhu, C. Expression, Purification and Characterization of Chondroitinase AC II from Marine Bacterium Arthrobacter sp. CS01. Mar. Drugs 2019, 17, 185.

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