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Mar. Drugs 2019, 17(2), 84;

Characteristics of a Novel Manganese Superoxide Dismutase of a Hadal Sea Cucumber (Paelopatides sp.) from the Mariana Trench

Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya 572000, China
College of Earth and Planetary Sciences, University of Chinese Academy of Sciences, Beijing 100039, China
Author to whom correspondence should be addressed.
Received: 17 December 2018 / Revised: 9 January 2019 / Accepted: 15 January 2019 / Published: 1 February 2019
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A novel, cold-adapted, and acid-base stable manganese superoxide dismutase (Ps-Mn-SOD) was cloned from hadal sea cucumber Paelopatides sp. The dimeric recombinant enzyme exhibited approximately 60 kDa in molecular weight, expressed activity from 0 °C to 70 °C with an optimal temperature of 0 °C, and resisted wide pH values from 2.2–13.0 with optimal activity (> 70%) at pH 5.0–12.0. The Km and Vmax of Ps-Mn-SOD were 0.0329 ± 0.0040 mM and 9112 ± 248 U/mg, respectively. At tested conditions, Ps-Mn-SOD was relatively stable in divalent metal ion and other chemicals, such as β-mercaptoethanol, dithiothreitol, Tween 20, Triton X-100, and Chaps. Furthermore, the enzyme showed striking stability in 5 M urea or 4 M guanidine hydrochloride, resisted digestion by proteases, and tolerated a high hydrostatic pressure of 100 MPa. The resistance of Ps-Mn-SOD against low temperature, extreme acidity and alkalinity, chemicals, proteases, and high pressure make it a potential candidate in biopharmaceutical and nutraceutical fields. View Full-Text
Keywords: expression; purification; deep-sea enzyme; pCold vector expression; purification; deep-sea enzyme; pCold vector

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Li, Y.; Kong, X.; Zhang, H. Characteristics of a Novel Manganese Superoxide Dismutase of a Hadal Sea Cucumber (Paelopatides sp.) from the Mariana Trench. Mar. Drugs 2019, 17, 84.

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