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Growth and Biochemical Composition of Porphyridium purpureum SCS-02 under Different Nitrogen Concentrations

CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, Institution of South China Sea Ecology and Environmental Engineering, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China
Author to whom correspondence should be addressed.
Mar. Drugs 2019, 17(2), 124;
Received: 18 January 2019 / Revised: 16 February 2019 / Accepted: 18 February 2019 / Published: 20 February 2019
(This article belongs to the Special Issue Bioactive Compounds Derived from Marine Microalgae)
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Microalgae of the genus Porphyridium show great potential for large-scale commercial cultivation, as they accumulate large quantities of B-phycoerythrin (B-PE), long chain polyunsaturated fatty acids (LC-PUFAs) and exopolysaccharide (EPS). The present study aimed to adjust culture nitrogen concentrations to produce Porphyridium biomass rich in B-PE, LC-PUFAs and EPS. Porphyridium purpureum SCS-02 was cultured in ASW culture medium with low nitrogen supply (LN, 3.5 mM), medium nitrogen supply (MN, 5.9 mM) or high nitrogen supply (HN, 17.6 mM). HN significantly enhanced the accumulation of biomass, intracellular protein, B-PE and eicosapentaenoic acid. LN increased the intracellular carbohydrate and arachidonic acid content, and promoted the secretion of EPS. The total lipids content was almost unaffected by nitrogen concentration. Based on these results, a semi-continuous two-step process was proposed, which included the production of biomass rich in B-PE and LC-PUFAs with sufficient nitrogen, and induced EPS excretion with limited nitrogen and strong light. View Full-Text
Keywords: Porphyridium; nitrogen; phycoerythrin; exopolysaccharide; polyunsaturated fatty acids Porphyridium; nitrogen; phycoerythrin; exopolysaccharide; polyunsaturated fatty acids

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Li, T.; Xu, J.; Wu, H.; Jiang, P.; Chen, Z.; Xiang, W. Growth and Biochemical Composition of Porphyridium purpureum SCS-02 under Different Nitrogen Concentrations. Mar. Drugs 2019, 17, 124.

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