Synergistic Anti-Candida Activity of Bengazole A in the Presence of Bengamide A†
AbstractBengazoles A–G from the marine sponge Jaspis sp. exhibit potent in vitro antifungal activity against Candida spp. and other pathogenic fungi. The mechanism of action (MOA) of bengazole A was explored in Candida albicans under both liquid culture and surface culture on Mueller-Hinton agar. Pronounced dose-dependent synergistic antifungal activity was observed with bengazole A in the presence of bengamide A, which is also a natural product from Jaspis sp. The MOA of bengazole A was further explored by monitoring the sterol composition of C. albicans in the presence of sub-lethal concentrations of bengazole A. The GCMS of solvent extracts prepared from liquid cultures of C. albicans in the presence of clotrimazole―a clinically approved azole antifungal drug that suppresses ergosterol biosynthesis by the inhibition of 14α-demethylase―showed reduced cellular ergosterol content and increased concentrations of lanosterol and 24-methylenedihydrolanosterol (a shunt metabolite of ergosterol biosynthesis). No change in relative sterol composition was observed when C. albicans was cultured with bengazole A. These results eliminate an azole-like MOA for the bengazoles, and suggest that another as-yet unidentified mechanism is operative. View Full-Text
- Supplementary File 1:
PDF-Document (PDF, 16628 KB)
Share & Cite This Article
Jamison, M.T.; Wang, X.; Cheng, T.; Molinski, T.F. Synergistic Anti-Candida Activity of Bengazole A in the Presence of Bengamide A. Mar. Drugs 2019, 17, 102.
Jamison MT, Wang X, Cheng T, Molinski TF. Synergistic Anti-Candida Activity of Bengazole A in the Presence of Bengamide A. Marine Drugs. 2019; 17(2):102.Chicago/Turabian Style
Jamison, Matthew T.; Wang, Xiao; Cheng, Tina; Molinski, Tadeusz F. 2019. "Synergistic Anti-Candida Activity of Bengazole A in the Presence of Bengamide A." Mar. Drugs 17, no. 2: 102.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.