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Open AccessArticle

Quantitative Structure-Activity Relationship Model to Predict Antioxidant Effects of the Peptide Fraction Extracted from a Co-Culture System of Chlorella pyrenoidosa and Yarrowia lipolytica

Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, China
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Author to whom correspondence should be addressed.
These authors have contributed equally to this work.
Mar. Drugs 2019, 17(11), 633; https://doi.org/10.3390/md17110633
Received: 8 October 2019 / Revised: 27 October 2019 / Accepted: 6 November 2019 / Published: 8 November 2019
(This article belongs to the Special Issue Strategies for Enhancing the Metabolome of Marine-Derived Fungi)
In this study, the antioxidant components in co-culture of Chlorella pyrenoidosa and Yarrowia lipolytica (3:1 ratio) were confirmed as trypsin-hydrolyzed peptides (EHPs). The EHPs were composed of 836 different peptides with molecular weights ranging from 639 to 3531 Da and were mainly composed of hydrophobic amino acids (48.1%). These peptides showed remarkable protective effects against oxidative stress in HepG2, which may be attributed to their structures. Furthermore, the mRNA and protein levels of nuclear factor erythroid 2-related factor 2 (Nrf2) were significantly lower in the peptide-treated group than in the control group, suggesting that the antioxidant enzyme-coding genes were not activated. The EC50 value of three peptides in the EHPs were in the order of AGYSPIGFVR (0.04 ± 0.002 mg/mL) > VLDELTLAR (0.09 ± 0.001 mg/mL) > LFDPVYLFDQG (0.41 ± 0.03 mg/mL); these results agreed with the prediction of the model (R2 > 0.9, Q2 > 0.5). Thus, EHPs show potential as potent new antioxidant agents. View Full-Text
Keywords: Chlorella pyrenoidosa; Yarrowia lipolytica; enzymatic hydrolysis peptides; HepG2; quantitative structure–activity relationship model Chlorella pyrenoidosa; Yarrowia lipolytica; enzymatic hydrolysis peptides; HepG2; quantitative structure–activity relationship model
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Liu, H.; Li, S.; Zhong, Y.; Liu, J.; Liu, H.; Cheng, J.; Ma, L.; Huang, Y.; Cai, X.; Liu, H.; Zheng, J.; Su, Z.; Wang, Q. Quantitative Structure-Activity Relationship Model to Predict Antioxidant Effects of the Peptide Fraction Extracted from a Co-Culture System of Chlorella pyrenoidosa and Yarrowia lipolytica. Mar. Drugs 2019, 17, 633.

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