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Marine Drugs
Volume 17
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10.3390/md17100589
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Article

Physicochemical and Biological Properties of Gelatin Extracted from Marine Snail Rapana venosa

by
Alexandra Gaspar-Pintiliescu
1,
Laura Mihaela Stefan
1,*,
Elena Daniela Anton
1,
Daniela Berger
2,
Cristian Matei
2,
Ticuta Negreanu-Pirjol
3 and
Lucia Moldovan
1
1
Departament of Cellular and Molecular Biology, National Institute of R&D for Biological Sciences, 296 Splaiul Independentei, 060031 Bucharest, Romania
2
Faculty of Applied Chemistry and Material Science, University “Politehnica” of Bucharest, 1-7 Gheorghe Polizu street, 011061 Bucharest, Romania
3
Faculty of Pharmacy, University “Ovidius” of Constanta, 1 Aleea Universitatii, 900470 Constanta, Romania
*
Author to whom correspondence should be addressed.
Mar. Drugs 2019, 17(10), 589; https://doi.org/10.3390/md17100589
Submission received: 26 July 2019 / Revised: 14 October 2019 / Accepted: 15 October 2019 / Published: 17 October 2019
(This article belongs to the Special Issue Marine Skeletal Biopolymers and Proteins, and Their Biomedical Application)
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Abstract

In this study, we aimed to obtain gelatin from the marine snail Rapana venosa using acidic and enzymatic extraction methods and to characterize these natural products for cosmetic and pharmaceutical applications. Marine gelatins presented protein values and hydroxyproline content similar to those of commercial mammalian gelatin, but with higher melting temperatures. Their electrophoretic profile and Fourier transform infrared (FTIR) spectra revealed protein and absorption bands situated in the amide region, specific for gelatin molecule. Scanning electron microscopy (SEM) analysis showed significant differences in the structure of the lyophilized samples, depending on the type of gelatin. In vitro studies performed on human keratinocytes showed no cytotoxic effect of acid-extracted gelatin at all tested concentrations and moderate cytotoxicity of enzymatic extracted gelatin at concentrations higher than 0.5 mg/mL. Also, both marine gelatins favored keratinocyte cell adhesion. No irritant potential was recorded as the level of IL-1α and IL-6 proinflammatory cytokines released by HaCaT cells cultivated in the presence of marine gelatins was significantly reduced. Together, these data suggest that marine snails are an alternative source of gelatins with potential use in pharmaceutical and skincare products.
Keywords: gelatin; marine gastropod; Black Sea; acidic and enzymatic extraction; biocompatibility; cytokines gelatin; marine gastropod; Black Sea; acidic and enzymatic extraction; biocompatibility; cytokines

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MDPI and ACS Style

Gaspar-Pintiliescu, A.; Stefan, L.M.; Anton, E.D.; Berger, D.; Matei, C.; Negreanu-Pirjol, T.; Moldovan, L. Physicochemical and Biological Properties of Gelatin Extracted from Marine Snail Rapana venosa. Mar. Drugs 2019, 17, 589. https://doi.org/10.3390/md17100589

AMA Style

Gaspar-Pintiliescu A, Stefan LM, Anton ED, Berger D, Matei C, Negreanu-Pirjol T, Moldovan L. Physicochemical and Biological Properties of Gelatin Extracted from Marine Snail Rapana venosa. Marine Drugs. 2019; 17(10):589. https://doi.org/10.3390/md17100589

Chicago/Turabian Style

Gaspar-Pintiliescu, Alexandra, Laura Mihaela Stefan, Elena Daniela Anton, Daniela Berger, Cristian Matei, Ticuta Negreanu-Pirjol, and Lucia Moldovan. 2019. "Physicochemical and Biological Properties of Gelatin Extracted from Marine Snail Rapana venosa" Marine Drugs 17, no. 10: 589. https://doi.org/10.3390/md17100589

APA Style

Gaspar-Pintiliescu, A., Stefan, L. M., Anton, E. D., Berger, D., Matei, C., Negreanu-Pirjol, T., & Moldovan, L. (2019). Physicochemical and Biological Properties of Gelatin Extracted from Marine Snail Rapana venosa. Marine Drugs, 17(10), 589. https://doi.org/10.3390/md17100589

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