Selective Inhibition of Liver Cancer Cells Using Venom Peptide
Abstract
:1. Introduction
2. Results
2.1. Tv1 Shows Selective Cytotoxicity in Liver Cancer Cells
2.2. Tv1 Induced Apoptotic Death of Liver Cancer Cells and Inhibited Migration
2.3. Tv1 Has Antitumor Activity in an Allograft Tumor Mouse Model
2.4. TRPC6 and TRPV6 Ion Channels Are Overexpressed in 1MEA Cells
2.5. Tv1 Co-Localizes with TRP Channel Subtypes
2.6. Tv1 Inhibited COX-2 Expression and Bioactivity
2.7. Molecular Modeling of the Tv1/TRPV6 Interaction
2.8. Mutational Analysis of the Tv1/TRPV6 Interaction
3. Discussion
4. Experimental Procedures
4.1. Tissue Culture and Reagents
4.2. Synthesis, Purification, and Characterization of Tv1
4.3. Fluorescent Labeling of Oxidized Tv1 Peptide
4.4. Synthesis and Rhodamine Labeling of Truncated Soricidin (TruncSorC) Peptide
4.5. Synthesis of a Non-Specific Fluorescent Peptide ACPK-Fam
4.6. In Vitro Cell Viability or MTT Assay
4.7. Flow Cytometry
4.8. Migration Assay
4.9. Antitumor Activity in Allograft Transplantation Models
4.10. In Cell Western Assay
4.11. Immunofluorescence Assay
4.12. Confocal Imaging and Colocalization Analyses
4.13. Analysis of Co-Localization using Manders Overlap Coefficient
4.14. Whole-cell Protein Extraction and Immunoblotting
4.15. Prostaglandin E2 (PGE2) Release
4.16. Statistical Analysis
4.17. Model Preparation and Docking
4.18. Molecular Dynamics (MD) Simulation
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Anand, P.; Filipenko, P.; Huaman, J.; Lyudmer, M.; Hossain, M.; Santamaria, C.; Huang, K.; Ogunwobi, O.O.; Holford, M. Selective Inhibition of Liver Cancer Cells Using Venom Peptide. Mar. Drugs 2019, 17, 587. https://doi.org/10.3390/md17100587
Anand P, Filipenko P, Huaman J, Lyudmer M, Hossain M, Santamaria C, Huang K, Ogunwobi OO, Holford M. Selective Inhibition of Liver Cancer Cells Using Venom Peptide. Marine Drugs. 2019; 17(10):587. https://doi.org/10.3390/md17100587
Chicago/Turabian StyleAnand, Prachi, Petr Filipenko, Jeannette Huaman, Michael Lyudmer, Marouf Hossain, Carolina Santamaria, Kelly Huang, Olorunseun O. Ogunwobi, and Mandë Holford. 2019. "Selective Inhibition of Liver Cancer Cells Using Venom Peptide" Marine Drugs 17, no. 10: 587. https://doi.org/10.3390/md17100587