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Mar. Drugs 2018, 16(8), 281;

Structure and Bioactivity Screening of a Low Molecular Weight Ulvan from the Green Alga Ulothrix flacca

Marine School, Ningbo University, 315000 Ningbo, China
Zhejiang Mariculture Research Institute, Zhoushan 316000, China
Shandong Institute of Food and Drug Control, Jinan 250000, China
College of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316000, China
Author to whom correspondence should be addressed.
Received: 26 June 2018 / Revised: 3 August 2018 / Accepted: 9 August 2018 / Published: 15 August 2018
(This article belongs to the Special Issue Discovery and Application of Macroalgae-Derived Natural Products)
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A water-soluble low molecular–weight polysaccharide named UP2-1 was isolated and purified from the marine green algae Ulothrix flacca using ion-exchange and size-exclusion chromatography. Composition and characteristics analyses showed that UP2-1 was a sulfated glucuronorhamnan consisting of rhamnose and glucuronic acid in a ratio of 2:1 with 21% sulfate content and a molecular weight of 5.0 kDa. Structural properties were determined using desulfation and methylation analyses combined with infrared spectrum (IR), gas chromatography-mass spectrometer (GC-MS) and nuclear magnetic resonance (NMR). The results showed that UP2-1 was a type of ulvan composed of alternate 4-linked-α-L-rhamnose residues (→4)-α-L-Rha(1→) and 4-linked-β-D-glucouronoc acid residues. The sulfate groups were mainly present in the O-3 position of →4)-α-L-Rha(1→. Most (70%) of the rhamnose was sulfated. UP2-1 also had a small amount of →4)-α-L-Rha(1→ branch at the O-2 position of the →4)-α-L-Rha(1→. UP2-1 exhibited significant anticoagulant and immunomodulating activity in vitro. This study demonstrated that the green algae Ulothrix flacca, which is used as a food and traditional marine herb in China, could also be considered as a source of bioactive ulvan. View Full-Text
Keywords: green algae; ulvan; structure; anticoagulant activity; immunoregulation green algae; ulvan; structure; anticoagulant activity; immunoregulation

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Li, P.; Wen, S.; Sun, K.; Zhao, Y.; Chen, Y. Structure and Bioactivity Screening of a Low Molecular Weight Ulvan from the Green Alga Ulothrix flacca. Mar. Drugs 2018, 16, 281.

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