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Mar. Drugs 2018, 16(8), 281; https://doi.org/10.3390/md16080281

Structure and Bioactivity Screening of a Low Molecular Weight Ulvan from the Green Alga Ulothrix flacca

1
Marine School, Ningbo University, 315000 Ningbo, China
2
Zhejiang Mariculture Research Institute, Zhoushan 316000, China
3
Shandong Institute of Food and Drug Control, Jinan 250000, China
4
College of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316000, China
*
Author to whom correspondence should be addressed.
Received: 26 June 2018 / Revised: 3 August 2018 / Accepted: 9 August 2018 / Published: 15 August 2018
(This article belongs to the Special Issue Discovery and Application of Macroalgae-Derived Natural Products)
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Abstract

A water-soluble low molecular–weight polysaccharide named UP2-1 was isolated and purified from the marine green algae Ulothrix flacca using ion-exchange and size-exclusion chromatography. Composition and characteristics analyses showed that UP2-1 was a sulfated glucuronorhamnan consisting of rhamnose and glucuronic acid in a ratio of 2:1 with 21% sulfate content and a molecular weight of 5.0 kDa. Structural properties were determined using desulfation and methylation analyses combined with infrared spectrum (IR), gas chromatography-mass spectrometer (GC-MS) and nuclear magnetic resonance (NMR). The results showed that UP2-1 was a type of ulvan composed of alternate 4-linked-α-L-rhamnose residues (→4)-α-L-Rha(1→) and 4-linked-β-D-glucouronoc acid residues. The sulfate groups were mainly present in the O-3 position of →4)-α-L-Rha(1→. Most (70%) of the rhamnose was sulfated. UP2-1 also had a small amount of →4)-α-L-Rha(1→ branch at the O-2 position of the →4)-α-L-Rha(1→. UP2-1 exhibited significant anticoagulant and immunomodulating activity in vitro. This study demonstrated that the green algae Ulothrix flacca, which is used as a food and traditional marine herb in China, could also be considered as a source of bioactive ulvan. View Full-Text
Keywords: green algae; ulvan; structure; anticoagulant activity; immunoregulation green algae; ulvan; structure; anticoagulant activity; immunoregulation
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Li, P.; Wen, S.; Sun, K.; Zhao, Y.; Chen, Y. Structure and Bioactivity Screening of a Low Molecular Weight Ulvan from the Green Alga Ulothrix flacca. Mar. Drugs 2018, 16, 281.

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