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Mar. Drugs 2018, 16(6), 191; https://doi.org/10.3390/md16060191

Heterologous Expression of a VioA Variant Activates Cryptic Compounds in a Marine-Derived Brevibacterium Strain

1,†
,
1,†
,
1
,
3
,
1,2,* and 1,2,*
1
Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China
2
Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China
3
Research Center for Marine Biology and Carbon Sequestration, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Biomass Energy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266003, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 17 May 2018 / Revised: 25 May 2018 / Accepted: 30 May 2018 / Published: 2 June 2018
(This article belongs to the Special Issue Microbial Gene Clusters of Marine Origin)
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Abstract

A new 14-membered homodimeric macrodiolide, brevidiolide (3), along with four known aromatic compounds (1, 2, 4 and 5) were obtained by heterologous expression of the recombinant plasmid pWLI823 expressing the G231L variant of VioA in the marine-derived Brevibacterium sp. 7002-073. The structures of 15 were elucidated on the basis of LC-MS and 2D NMR spectroscopic analyses. In the evaluation for the antibacterial activities of the compounds against multi-drug resistant (MDR) strains, 5 showed notable growth inhibition against Staphylococcus aureus CCARM 3090 and Klebsiella pneumoniae ATCC 13883, with a minimum inhibitory concentration (MIC) value of 3.12 µg/mL. View Full-Text
Keywords: heterologous expression; recombinant; Brevibacterium; macrodiolide; antibacterial heterologous expression; recombinant; Brevibacterium; macrodiolide; antibacterial
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Han, X.; Hou, L.; Hou, J.; Zhang, Y.; Li, H.; Li, W. Heterologous Expression of a VioA Variant Activates Cryptic Compounds in a Marine-Derived Brevibacterium Strain. Mar. Drugs 2018, 16, 191.

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