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Open AccessArticle

Heterologous Expression of a VioA Variant Activates Cryptic Compounds in a Marine-Derived Brevibacterium Strain

by 1,†, 1,†, 1, 3, 1,2,* and 1,2,*
1
Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China
2
Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China
3
Research Center for Marine Biology and Carbon Sequestration, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Biomass Energy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266003, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Mar. Drugs 2018, 16(6), 191; https://doi.org/10.3390/md16060191
Received: 17 May 2018 / Revised: 25 May 2018 / Accepted: 30 May 2018 / Published: 2 June 2018
(This article belongs to the Special Issue Microbial Gene Clusters of Marine Origin)
A new 14-membered homodimeric macrodiolide, brevidiolide (3), along with four known aromatic compounds (1, 2, 4 and 5) were obtained by heterologous expression of the recombinant plasmid pWLI823 expressing the G231L variant of VioA in the marine-derived Brevibacterium sp. 7002-073. The structures of 15 were elucidated on the basis of LC-MS and 2D NMR spectroscopic analyses. In the evaluation for the antibacterial activities of the compounds against multi-drug resistant (MDR) strains, 5 showed notable growth inhibition against Staphylococcus aureus CCARM 3090 and Klebsiella pneumoniae ATCC 13883, with a minimum inhibitory concentration (MIC) value of 3.12 µg/mL. View Full-Text
Keywords: heterologous expression; recombinant; Brevibacterium; macrodiolide; antibacterial heterologous expression; recombinant; Brevibacterium; macrodiolide; antibacterial
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MDPI and ACS Style

Han, X.; Hou, L.; Hou, J.; Zhang, Y.; Li, H.; Li, W. Heterologous Expression of a VioA Variant Activates Cryptic Compounds in a Marine-Derived Brevibacterium Strain. Mar. Drugs 2018, 16, 191.

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Mar. Drugs, EISSN 1660-3397, Published by MDPI AG
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