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Open AccessArticle

Crambescidin 800, Isolated from the Marine Sponge Monanchora viridis, Induces Cell Cycle Arrest and Apoptosis in Triple-Negative Breast Cancer Cells

1
School of Molecular Sciences, The University of Western Australia, Crawley 6009, Western Australia, Australia
2
Cancer Epigenetics, Harry Perkins Institute of Medical Research, QEII Medical Centre and Centre for Medical Research, The University of Western Australia, Crawley 6009, Western Australia, Australia
3
Western Australian Museum, Welshpool 6106, Western Australia, Australia
*
Authors to whom correspondence should be addressed.
Mar. Drugs 2018, 16(2), 53; https://doi.org/10.3390/md16020053
Received: 7 December 2017 / Revised: 22 January 2018 / Accepted: 31 January 2018 / Published: 8 February 2018
(This article belongs to the Special Issue Bioactive Compounds from Marine Sponges)
Triple negative breast cancer (TNBC) is currently the only group of breast cancers without an effective targeted therapy. Marine sponges have historically been a source of compounds with anticancer activity. In this study, we screened extracts from twenty marine sponges collected off the coast of Western Australia for cytotoxic activity against TNBC cells. One very active extract derived from the sponge Monanchora viridis was selected for bioactivity-guided fractionation. Through multiple steps of purification, we isolated a potent cytotoxic compound, which was identified as crambescidin 800 (C800). We found that C800 exhibited cytotoxic potency in a panel of breast cancer cells, of which TNBC and luminal cancer cell models were the most sensitive. In addition, C800 induced cell cycle arrest at the G2/M phase, resulting in a decline in the expression of cyclin D1, CDK4, and CDK6 in TNBC cells. This effect was associated with the inhibition of phosphorylation of Akt, NF-κB, and MAPK pathways, resulting in apoptosis in TNBC cells. View Full-Text
Keywords: crambescidin 800; triple-negative breast cancer; marine sponge crambescidin 800; triple-negative breast cancer; marine sponge
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MDPI and ACS Style

Shrestha, S.; Sorolla, A.; Fromont, J.; Blancafort, P.; Flematti, G.R. Crambescidin 800, Isolated from the Marine Sponge Monanchora viridis, Induces Cell Cycle Arrest and Apoptosis in Triple-Negative Breast Cancer Cells. Mar. Drugs 2018, 16, 53. https://doi.org/10.3390/md16020053

AMA Style

Shrestha S, Sorolla A, Fromont J, Blancafort P, Flematti GR. Crambescidin 800, Isolated from the Marine Sponge Monanchora viridis, Induces Cell Cycle Arrest and Apoptosis in Triple-Negative Breast Cancer Cells. Marine Drugs. 2018; 16(2):53. https://doi.org/10.3390/md16020053

Chicago/Turabian Style

Shrestha, Sumi; Sorolla, Anabel; Fromont, Jane; Blancafort, Pilar; Flematti, Gavin R. 2018. "Crambescidin 800, Isolated from the Marine Sponge Monanchora viridis, Induces Cell Cycle Arrest and Apoptosis in Triple-Negative Breast Cancer Cells" Mar. Drugs 16, no. 2: 53. https://doi.org/10.3390/md16020053

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