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Mar. Drugs 2018, 16(12), 495; https://doi.org/10.3390/md16120495

Evaluation of the Potential of Collagen from Codfish Skin as a Biomaterial for Biomedical Applications

1
3B’s Research Group, I3Bs–Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence in Tissue Engineering and Regenerative Medicine Avepark–Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco, Guimarães, Portugal
2
ICVS/3B’s–PT Government Associate Laboratory, 4805-017 Braga/Guimarães, Portugal
3
The Discoveries Centre for Regenerative and Precision Medicine, Headquarters at University of Minho, Avepark, 4805-017 Barco, Guimarães, Portugal
*
Author to whom correspondence should be addressed.
Received: 6 November 2018 / Revised: 30 November 2018 / Accepted: 5 December 2018 / Published: 8 December 2018
(This article belongs to the Special Issue Collagen from Marine Biological Source and Medical Applications)
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Abstract

Collagen is one of the most widely used biomaterials, not only due its biocompatibility, biodegradability and weak antigenic potential, but also due to its role in the structure and function of tissues. Searching for alternative collagen sources, the aim of this study was to extract collagen from the skin of codfish, previously obtained as a by-product of fish industrial plants, and characterize it regarding its use as a biomaterial for biomedical application, according to American Society for Testing and Materials (ASTM) Guidelines. Collagen type I with a high degree of purity was obtained through acid-extraction, as confirmed by colorimetric assays, SDS-PAGE and amino acid composition. Thermal analysis revealed a denaturing temperature around 16 °C. Moreover, collagen showed a concentration-dependent effect in metabolism and on cell adhesion of lung fibroblast MRC-5 cells. In conclusion, this study shows that collagen can be obtained from marine-origin sources, while preserving its bioactivity, supporting its use in biomedical applications. View Full-Text
Keywords: marine-origin collagen; codfish; biophysical characterization; biologic activity; ASTM guidelines; biomedical application; marine biomaterials marine-origin collagen; codfish; biophysical characterization; biologic activity; ASTM guidelines; biomedical application; marine biomaterials
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Carvalho, A.M.; Marques, A.P.; Silva, T.H.; Reis, R.L. Evaluation of the Potential of Collagen from Codfish Skin as a Biomaterial for Biomedical Applications. Mar. Drugs 2018, 16, 495.

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