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Mar. Drugs 2018, 16(10), 370; https://doi.org/10.3390/md16100370

Characterization of a Novel N-Acylhomoserine Lactonase RmmL from Ruegeria mobilis YJ3

1
College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
2
College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China
3
Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China
*
Author to whom correspondence should be addressed.
Received: 19 August 2018 / Revised: 27 September 2018 / Accepted: 1 October 2018 / Published: 8 October 2018
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Abstract

Gram-negative bacteria utilize N-acylhomoserine lactones (AHLs) as quorum sensing (QS) signaling molecules for intercellular communication. Cell-to-cell communication depends on cell population density, and AHL-dependent QS is related to the production of multiple genes including virulence factors. Quorum quenching (QQ), signal inactivation by enzymatic degradation, is a potential strategy for attenuating QS regulated bacterial infections. Both Gram-positive and -negative bacteria have QQ enzymes that can degrade AHLs. In our previous study, strain Ruegeria mobilis YJ3, isolated from healthy shrimp, showed strong AHLs degradative activity. In the current study, an AHL lactonase (designated RmmL) was cloned and characterized from Ruegeria mobilis YJ3. Amino acid sequence analysis showed that RmmL has a conserved “HXHXDH” motif and clusters together with lactonase AidC that belongs to the metallo-β-lactamase superfamily. Recombinant RmmL could degrade either short- or long-chain AHLs in vitro. High-performance liquid chromatography analysis indicated that RmmL works as an AHL lactonase catalyzing AHL ring-opening by hydrolyzing lactones. Furthermore, RmmL can reduce the production of pyocyanin by Pseudomonas aeruginosa PAO1, while for the violacein and the extracellular protease activities by Chromobacterium violaceum CV026 and Vibrio anguillarum VIB72, no significant reduction was observed. This study suggests that RmmL might be used as a therapeutic agent in aquaculture. View Full-Text
Keywords: N-acylhomoserine lactonase; quorum quenching; Ruegeria mobilis; RmmL N-acylhomoserine lactonase; quorum quenching; Ruegeria mobilis; RmmL
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Cai, X.; Yu, M.; Shan, H.; Tian, X.; Zheng, Y.; Xue, C.; Zhang, X.-H. Characterization of a Novel N-Acylhomoserine Lactonase RmmL from Ruegeria mobilis YJ3. Mar. Drugs 2018, 16, 370.

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