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Suppression of RANKL-Induced Osteoclastogenesis by the Metabolites from the Marine Fungus Aspergillus flocculosus Isolated from a Sponge Stylissa sp.

1
Marine Natural Products Chemistry Laboratory, Korea Institute of Ocean Science and Technology, 385, Haeyang-ro, Yeongdo-gu, Busan Metropolitan City 49111, Korea
2
Department of Marine Biotechnology, University of Science and Technology, 217 Gajungro Yuseong-gu, Daejeon 34113, Korea
3
Nhatrang Institute of Technology Research and Application, Vietnam Academy of Science and Technology, 02 Hung Vuong, Nha Trang 650000, Vietnam
4
Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi 100000, Vietnam
5
Bio-Evaluation Center, Korea Research Institute of Biotechnology, 30 Yeongudanjiro, Cheongju 28116, Korea
*
Author to whom correspondence should be addressed.
Mar. Drugs 2018, 16(1), 14; https://doi.org/10.3390/md16010014
Received: 3 November 2017 / Revised: 29 November 2017 / Accepted: 8 December 2017 / Published: 5 January 2018
A new α-pyrone merosesquiterpenoid possessing an angular tetracyclic carbon skeleton, ochraceopone F (1), and four known secondary metabolites, aspertetranone D (2), cycloechinulin (3), wasabidienone E (4), and mactanamide (5), were isolated from the marine fungus Aspergillus flocculosus derived from a sponge Stylissa sp. collected in Vietnam. The structures of Compounds 15 were elucidated by analysis of 1D and 2D NMR spectra and MS data. All the isolated compounds were evaluated for anti-proliferation activity and their suppression effects on receptor activator of nuclear factor κB ligand (RANKL)-induced osteoclast differentiation using tartate-resisant acid phosphatase (TRAP). Compounds 15 had no anti-proliferative effect on human cancer cell lines up to 30 μg/mL. Among these compounds, aspertetranone D (2) and wasabidienone E (4) exhibited weak osteoclast differentiation inhibitory activity at 10 μg/mL. However, mactanamide (5) showed a potent suppression effect of osteoclast differentiation without any evidence of cytotoxicity. View Full-Text
Keywords: mactanamide; osteoclastogenesis; marine fungus; Aspergillus flocculosus; TRAP assay mactanamide; osteoclastogenesis; marine fungus; Aspergillus flocculosus; TRAP assay
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Shin, H.J.; Choi, B.-K.; Trinh, P.T.H.; Lee, H.-S.; Kang, J.S.; Van, T.T.T.; Lee, H.-S.; Lee, J.S.; Lee, Y.-J.; Lee, J. Suppression of RANKL-Induced Osteoclastogenesis by the Metabolites from the Marine Fungus Aspergillus flocculosus Isolated from a Sponge Stylissa sp.. Mar. Drugs 2018, 16, 14.

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