Bacillamidins A–G from a Marine-Derived Bacillus pumilus
Abstract
:1. Introduction
2. Results and Discussion
3. Materials and Methods
3.1. General Experimental Procedures
3.2. Strain and Cultivation
3.3. Extraction and Isolation
3.4. ECD Calculation Methods
3.5. Bioassay
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Position | 1 | 2 | ||
---|---|---|---|---|
δH (J, Hz) | δC, Type | δH (J, Hz) | δC, Type | |
1-NH | 8.31, d (7.6) | 8.42, d (7.6) | ||
2 | 172.2, CO | 172.2, CO | ||
3 | 2.08, t (7.6) | 34.9, CH2 | 2.08, t (7.6) | 34.9, CH2 |
4 | 1.48, m | 25.1, CH2 | 1.47, m | 25.1, CH2 |
5 | 1.23, m | 28.4, CH2 | 1.23, m | 28.4, CH2 |
6 | 1.23, m | 28.7, CH2 | 1.23, m | 28.7, CH2 |
7 | 1.23, m | 29.2, CH2 | 1.23, m | 28.9, CH2 |
8 | 1.23, m | 26.7, CH2 | 1.23, m | 29.3, CH2 |
9 | 1.13, m | 38.4, CH2 | 1.23, m | 26.4, CH2 |
10 | 1.48, m | 27.4, CH | 1.09, m; 1.27, m | 36.0, CH2 |
11 | 0.84, d (6.4) | 22.5, CH3 | 1.30, m | 33.7, CH |
12 | 0.84, d (6.4) | 22.5, CH3 | 1.10, m | 29.0, CH2 |
13 | 0.83, t (9.0) | 11.2, CH3 | ||
14 | 0.78, d (9.0) | 19.1, CH3 | ||
1′ | 4.60, ddd (9.6, 7.6, 6.0) | 48.4, CH | 4.60, ddd (9.6, 7.6, 6.0) | 48.4, CH |
2′ | 2.79, dd (16.4, 9.6) | 35.6, CH2 | 2.79, dd (16.4, 9.6) | 35.6, CH2 |
2.67, dd (16.4, 6.0) | 2.67, dd (16.4, 6.0) | |||
3′ | 171.2, CO | 171.3, CO | ||
3′-OCH3 | 3.61, s | 51.6, OCH3 | 3.61, s | 51.6, OCH3 |
1″ | 170.4, CO | 170.4, CO | ||
1″-OCH3 | 3.60, s | 52.0, OCH3 | 3.60, s | 52.1, OCH3 |
Position | 3 | 4 | ||
---|---|---|---|---|
δH (J, Hz) | δC, Type | δH (J, Hz) | δC, Type | |
1-NH | 8.42, d (7.6) | 8.51, d (7.6) | ||
2 | 172.5, CO | 172.6, CO | ||
3 | 2.08, t (7.6) | 34.9, CH2 | 2.08, t (7.6) | 34.8, CH2 |
4 | 1.23, m | 25.0, CH2 | 1.48, m | 25.0, CH2 |
5 | 1.23, m | 28.5, CH2 | 1.23, m | 28.4, CH2 |
6 | 1.23, m | 28.8, CH2 | 1.23, m | 28.8, CH2 |
7 | 1.23, m | 29.0, CH2 | 1.23, m | 29.2, CH2 |
8 | 1.23, m | 29.3, CH2 | 1.23, m | 26.7, CH2 |
9 | 1.23, m | 26.5, CH2 | 1.13, m | 38.4, CH2 |
10 | 1.09, m; 1.27, m | 36.2, CH2 | 1.48, m | 27.4, CH |
11 | 1.30, m | 33.7, CH | 0.83, d (6.6) | 22.5, CH3 |
12 | 1.10, m | 29.0, CH2 | 0.83, d (6.6) | 22.5, CH3 |
13 | 0.83, t (9.0) | 11.2, CH3 | ||
14 | 0.78, d (9.0) | 19.1, CH3 | ||
1′ | 4.35, ddd (9.6, 7.6, 6.0) | 49.5, CH | 4.35, ddd (9.6, 7.6, 6.0) | 48.4, CH |
2′ | 177.7, CO | 176.5, CO | ||
3′-NH | 11.19, s | |||
4′ | 176.4, CO | 175.2, CO | ||
5′ | 2.84, dd (16.4, 9.6) | 36.0, CH2 | 2.91, dd (16.4, 9.6) | 34.8, CH2 |
2.53, dd (16.4, 6.0) | 2.53, dd (16.4, 6.0) | |||
1″ | 3.19, d (7.2) | 45.4, CH2 | ||
2″ | 1.90, m | 26.7, CH | ||
3″ | 0.84, d (6.6) | 20.0, CH3 | ||
4″ | 0.84, d (6.6) | 20.0, CH3 |
Position | 5 | ||||
---|---|---|---|---|---|
δH (J, Hz) | δC, Type | Position | δH (J, Hz) | δC, Type | |
1-NH | 8.34, d (6.8) | 1″ | 0.80, d (6.4) | 21.3, CH3 | |
2 | 171.9, CO | 2″ | 0.88, d (6.4) | 23.3, CH3 | |
3 | 1.90, t (7.6) | 35.0, CH2 | 3″ | 1.56, m | 24.0, CH |
4 | 1.48, m | 24.9, CH2 | 4″ | 1.32, m | 38.4, CH2 |
5-10 | 1.25, m | 28.5–29.2 | 1.68, m | ||
11 | 1.25, m | 29.3, CH2 | 5″ | 4.19, dddd (3.6, 7.6, 10.4, 13.6) | 48.3, CH |
12 | 1.25, m | 26.7, CH2 | 6″-NH | 7.94, d (9.6) | |
13 | 1.13, m | 38.4, CH2 | 7″ | 169.8, CO | |
14 | 1.49, m | 27.4, CH | 8″ | 4.27, dd (6.0, 2.4) | 71.6, CH |
15 | 0.83, d, (6.6) | 22.5, CH3 | 8″-OH | 6.18, d (6.0) | |
16 | 0.83, d, (6.6) | 22.5, CH3 | 9″ | 4.61, dd (2.4, 2.0) | 85.7, CH |
1′ | 168.9, CO | 10″ | 4.32, m | 46.1, CH | |
3′ | 4.69, ddd (10.4, 8.0, 2.8) | 80.8, CH | 11″ | 2.16, dd (18.0, 2.2) | 36.0, CH2 |
4′ | 2.87, m | 29.0, CH2 | 2.85, m | ||
5′ | 6.82, d (7.6) | 118.5, CH | 12″ | 175.6, CO | |
6′ | 7.48, dd (8.4, 7.6) | 136.2, CH | |||
7′ | 6.84, d (8.4) | 115.2, CH | |||
8′ | 160.8, C | ||||
8″-OH | 10.79, s | ||||
9′ | 108.3, C | ||||
10′ | 140.4, C |
Compounds | P. aeruginosa PA-01 | A. baumannii ATCC19606 | E. coli BW25113 |
---|---|---|---|
1 | 64 | 58 | >128 |
2 | 64 | 64 | >128 |
3 | 64 | 64 | >128 |
4 | 64 | 58 | >128 |
5 | >128 | >128 | >128 |
6 | >128 | >128 | >128 |
7 | >128 | >128 | >128 |
ofloxacin | 8 | 16 | 1 |
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Zhou, S.-Y.; Hu, Y.-J.; Meng, F.-C.; Qu, S.-Y.; Wang, R.; Andersen, R.J.; Liao, Z.-H.; Chen, M. Bacillamidins A–G from a Marine-Derived Bacillus pumilus. Mar. Drugs 2018, 16, 326. https://doi.org/10.3390/md16090326
Zhou S-Y, Hu Y-J, Meng F-C, Qu S-Y, Wang R, Andersen RJ, Liao Z-H, Chen M. Bacillamidins A–G from a Marine-Derived Bacillus pumilus. Marine Drugs. 2018; 16(9):326. https://doi.org/10.3390/md16090326
Chicago/Turabian StyleZhou, Si-Yu, Yi-Jie Hu, Fan-Cheng Meng, Shen-Yue Qu, Rui Wang, Raymond J. Andersen, Zhi-Hua Liao, and Min Chen. 2018. "Bacillamidins A–G from a Marine-Derived Bacillus pumilus" Marine Drugs 16, no. 9: 326. https://doi.org/10.3390/md16090326
APA StyleZhou, S.-Y., Hu, Y.-J., Meng, F.-C., Qu, S.-Y., Wang, R., Andersen, R. J., Liao, Z.-H., & Chen, M. (2018). Bacillamidins A–G from a Marine-Derived Bacillus pumilus. Marine Drugs, 16(9), 326. https://doi.org/10.3390/md16090326