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Marine Drugs
  • Correction
  • Open Access

9 September 2015

Dolch, L.-J. and Maréchal, E. Inventory of Fatty Acid Desaturases in the Pennate Diatom Phaeodactylum tricornutum. Mar. Drugs 2015, 13, 1317–1339

and
Laboratory of Plant and Cell Physiology/Laboratoire de Physiologie Cellulaire et Végétale, Unité mixte de recherche 5168 CNRS-CEA-Université Grenoble Alpes, Institut de Recherche en Sciences et Technologies pour le Vivant, CEA Grenoble, 17 rue des Martyrs, 38054 Grenoble Cedex 9, France
*
Author to whom correspondence should be addressed.
This article belongs to the Special Issue Metabolites in Diatoms
The authors wish to make the following corrections to this paper [1]:
We have found eight inadvertent errors in our paper published in Mar. Drugs [1].
On page 1327, Line 6 and column 9 of Table 2, “18:3Δ6,12,15” should be “18:3Δ6,9,12”. Line 8 and column 8 of Table 2, “20:4Δ5,8,11,14,17” should be “20:4Δ8,11,14,17”. Line 9 and column 7, “Δ5/cis” should be “Δ4/cis”. Therefore replace this incorrect table:
Table 2. Fatty acid desaturases of Phaeodactylum tricornutum—Characterized or predicted localization and substrate specificity. (*) functionally characterized; (?) based on predictions.
Table 2. Fatty acid desaturases of Phaeodactylum tricornutum—Characterized or predicted localization and substrate specificity. (*) functionally characterized; (?) based on predictions.
NameGene IDSubcellular LocalizationMain Substrate Main Product
Acyl Linked to:Carbon NumberPresence of Double BondsPosition and Configuration of the Introduced Double BondOverall StructureOverall Structure
PAD/SADPhatraft_9316Chloroplast stromaACP16 and 180Δ9/cis16:0-ACP16:1Δ9-ACP
ADSPhatr_28797Endomembrane systemCoA18?0Δ9/cis?18:0-CoA?18:1Δ9-CoA?
FAD2 (*)Phatr_25769ERPhospholipid/
Betaine lipid?
181Δ12 (or ω6)/cis18:1Δ9-PL (-BL?)18:2Δ9,12-PL (-BL?)
ERΔ6FAD (*)Phatr_2948ERPhospholipid/
Betaine lipid?
182Δ6/cis18:2Δ9,12-PL (-BL?)18:3Δ6,12,15-PL (-BL?)
ERω3FAD (?)?ERPhospholipid/
Betaine lipid?
183Δ15 (or ω3)/cis18:3Δ6,9,12-PL (-BL?)18:4Δ6,9,12,15-PL (-BL?)
ERΔ5FAD.1 (*)
ERΔ5FAD.2 (?)
Phatr_46830 Phatr_22459ERPhospholipid/
Betaine lipid?
204Δ5/cis20:4Δ5,8,11,14,17-PL (-BL?)20:5Δ5,8,11,14,17-PL (-BL?)
ERΔ4FADPhatr_22510?ERPhospholipid/
Betaine lipid?
225Δ5/cis22:5Δ7,10,13,16,19-PL (-BL?)22:6Δ4,7,10,13,16,19-PL (-BL?)
FAD6 (*)Phatr_48423Chloroplast membranessn1/sn2-MGDG/DGDG + SQDG161Δ12/cis16:1Δ9-sn2-MGDG/DGDG/SQDG16:2Δ9,12-sn1/sn2-MGDG/DGDG/SQDG
PlastidΔ6FAD (?)Phatr_50443Chloroplast membranessn2-MGDG162Δ6/cis16:2Δ9,12-sn2-MGDG16:3Δ6,9,12-sn2-MGDG
Plastidω3FAD/FAD7 (?)Phatr_41570Chloroplast membranessn2-MGDG?163ω3/cis16:3Δ6,9,12-sn2-MGDG16:4Δ6,9,12,15-sn2-MGDG
FAD4Phatr_41301Chloroplast membranessn2-PG160Δ3/trans16:0-sn2-PG16:1Δ3trans-sn2-PG
With the following corrected table:
Table 2. Fatty acid desaturases of Phaeodactylum tricornutum—Characterized or predicted localization and substrate specificity. (*) functionally characterized; (?) based on predictions.
Table 2. Fatty acid desaturases of Phaeodactylum tricornutum—Characterized or predicted localization and substrate specificity. (*) functionally characterized; (?) based on predictions.
NameGene IDSubcellular LocalizationMain Substrate Main Product
Acyl Linked to:Carbon NumberPresence of Double BondsPosition and Configuration of the Introduced Double BondOverall StructureOverall Structure
PAD/SADPhatraft_9316Chloroplast stromaACP16 and 180Δ9/cis16:0-ACP16:1Δ9-ACP
ADSPhatr_28797Endomembrane systemCoA18?0Δ9/cis?18:0-CoA?18:1Δ9-CoA?
FAD2 (*)Phatr_25769ERPhospholipid/
Betaine lipid?
181Δ12 (or ω6)/cis18:1Δ9-PL (-BL?)18:2Δ9,12-PL (-BL?)
ERΔ6FAD (*)Phatr_2948ERPhospholipid/
Betaine lipid?
182Δ6/cis18:2Δ9,12-PL (-BL?)18:3Δ6,9,12-PL (-BL?)
ERω3FAD (?)?ERPhospholipid/
Betaine lipid?
183Δ15 (or ω3)/cis18:3Δ6,9,12-PL (-BL?)18:4Δ6,9,12,15-PL (-BL?)
ERΔ5FAD.1 (*)
ERΔ5FAD.2 (?)
Phatr_46830
Phatr_22459
ERPhospholipid/
Betaine lipid?
204Δ5/cis20:4Δ8,11,14,17-PL (-BL?)20:5Δ5,8,11,14,17-PL (-BL?)
ERΔ4FADPhatr_22510?ERPhospholipid/
Betaine lipid?
225Δ4/cis22:5Δ7,10,13,16,19-PL (-BL?)22:6Δ4,7,10,13,16,19-PL (-BL?)
FAD6 (*)Phatr_48423Chloroplast membranessn1/sn2-MGDG/DGDG + SQDG161Δ12/cis16:1Δ9-sn2-MGDG/DGDG/SQDG16:2Δ9,12-sn1/sn2-MGDG/DGDG/SQDG
PlastidΔ6FAD (?)Phatr_50443Chloroplast membranessn2-MGDG162Δ6/cis16:2Δ9,12-sn2-MGDG16:3Δ6,9,12-sn2-MGDG
Plastidω3FAD/FAD7 (?)Phatr_41570Chloroplast membranessn2-MGDG?163ω3/cis16:3Δ6,9,12-sn2-MGDG16:4Δ6,9,12,15-sn2-MGDG
FAD4Phatr_41301Chloroplast membranessn2-PG160Δ3/trans16:0-sn2-PG16:1Δ3trans-sn2-PG
On page 1330, line 9, “18:3 and 14” should be “18:3 and 18:4”. Line 29, in the title of Section 2.4.10, “The (18:2Δ9,12 → 18:3Δ6,9,12 and 18:3Δ9,12,15 → 18:3Δ6,9,12,15)-Desaturation” should be “The (18:2Δ9,12 → 18:3Δ6,9,12 and 18:3Δ9,12,15 → 18:4Δ6,9,12,15)-Desaturation”. On page 1331, line 6, in the title of Section 2.4.11, “The Question of the (18:2Δ9,12 → 18:3Δ6,9,15 and 18:3Δ6,9,12 → 18:4Δ6,9,12,15)-Desaturation” should be “The Question of the (18:2Δ9,12 → 18:3Δ9,12,15 and 18:3Δ6,9,12 → 18:4Δ6,9,12,15)-Desaturation”. On page 1332, line 1, “E Δ6FAD” should be “ERΔ6FAD”. On page 1335, lines 25–26, in the Conclusions Section, “ERD6FAD, and ERD5FAD” should be “ERΔ6FAD, and ERΔ5FAD”.
These changes have no material impact on the conclusions of our paper. We apologize to our readers for any inconvenience caused.

Reference

  1. Dolch, L.J.; Maréchal, E. Inventory of fatty acid desaturases in the pennate diatom Phaeodactylum tricornutum. Mar. Drugs 2015, 13, 1317–1339. [Google Scholar] [CrossRef] [PubMed]

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