Dolch, L.-J. and Maréchal, E. Inventory of Fatty Acid Desaturases in the Pennate Diatom Phaeodactylum tricornutum. Mar. Drugs 2015, 13, 1317–1339
Laboratory of Plant and Cell Physiology/Laboratoire de Physiologie Cellulaire et Végétale, Unité mixte de recherche 5168 CNRS-CEA-Université Grenoble Alpes, Institut de Recherche en Sciences et Technologies pour le Vivant, CEA Grenoble, 17 rue des Martyrs, 38054 Grenoble Cedex 9, France
*
Author to whom correspondence should be addressed.
Mar. Drugs 2015, 13(9), 5732-5735; https://doi.org/10.3390/md13095732
Submission received: 26 May 2015
/
Accepted: 27 May 2015
/
Published: 9 September 2015
(This article belongs to the Special Issue Metabolites in Diatoms)
The authors wish to make the following corrections to this paper [1]:
We have found eight inadvertent errors in our paper published in Mar. Drugs [1].
On page 1327, Line 6 and column 9 of Table 2, “18:3Δ6,12,15” should be “18:3Δ6,9,12”. Line 8 and column 8 of Table 2, “20:4Δ5,8,11,14,17” should be “20:4Δ8,11,14,17”. Line 9 and column 7, “Δ5/cis” should be “Δ4/cis”. Therefore replace this incorrect table:
Table 2.
Fatty acid desaturases of Phaeodactylum tricornutum—Characterized or predicted localization and substrate specificity. (*) functionally characterized; (?) based on predictions.
| Name | Gene ID | Subcellular Localization | Main Substrate | Main Product | ||||
|---|---|---|---|---|---|---|---|---|
| Acyl Linked to: | Carbon Number | Presence of Double Bonds | Position and Configuration of the Introduced Double Bond | Overall Structure | Overall Structure | |||
| PAD/SAD | Phatraft_9316 | Chloroplast stroma | ACP | 16 and 18 | 0 | Δ9/cis | 16:0-ACP | 16:1Δ9-ACP |
| ADS | Phatr_28797 | Endomembrane system | CoA | 18? | 0 | Δ9/cis? | 18:0-CoA? | 18:1Δ9-CoA? |
| FAD2 (*) | Phatr_25769 | ER | Phospholipid/ Betaine lipid? | 18 | 1 | Δ12 (or ω6)/cis | 18:1Δ9-PL (-BL?) | 18:2Δ9,12-PL (-BL?) |
| ERΔ6FAD (*) | Phatr_2948 | ER | Phospholipid/ Betaine lipid? | 18 | 2 | Δ6/cis | 18:2Δ9,12-PL (-BL?) | 18:3Δ6,12,15-PL (-BL?) |
| ERω3FAD (?) | ? | ER | Phospholipid/ Betaine lipid? | 18 | 3 | Δ15 (or ω3)/cis | 18:3Δ6,9,12-PL (-BL?) | 18:4Δ6,9,12,15-PL (-BL?) |
| ERΔ5FAD.1 (*) ERΔ5FAD.2 (?) | Phatr_46830 Phatr_22459 | ER | Phospholipid/ Betaine lipid? | 20 | 4 | Δ5/cis | 20:4Δ5,8,11,14,17-PL (-BL?) | 20:5Δ5,8,11,14,17-PL (-BL?) |
| ERΔ4FAD | Phatr_22510? | ER | Phospholipid/ Betaine lipid? | 22 | 5 | Δ5/cis | 22:5Δ7,10,13,16,19-PL (-BL?) | 22:6Δ4,7,10,13,16,19-PL (-BL?) |
| FAD6 (*) | Phatr_48423 | Chloroplast membranes | sn1/sn2-MGDG/DGDG + SQDG | 16 | 1 | Δ12/cis | 16:1Δ9-sn2-MGDG/DGDG/SQDG | 16:2Δ9,12-sn1/sn2-MGDG/DGDG/SQDG |
| PlastidΔ6FAD (?) | Phatr_50443 | Chloroplast membranes | sn2-MGDG | 16 | 2 | Δ6/cis | 16:2Δ9,12-sn2-MGDG | 16:3Δ6,9,12-sn2-MGDG |
| Plastidω3FAD/FAD7 (?) | Phatr_41570 | Chloroplast membranes | sn2-MGDG? | 16 | 3 | ω3/cis | 16:3Δ6,9,12-sn2-MGDG | 16:4Δ6,9,12,15-sn2-MGDG |
| FAD4 | Phatr_41301 | Chloroplast membranes | sn2-PG | 16 | 0 | Δ3/trans | 16:0-sn2-PG | 16:1Δ3trans-sn2-PG |
With the following corrected table:
Table 2.
Fatty acid desaturases of Phaeodactylum tricornutum—Characterized or predicted localization and substrate specificity. (*) functionally characterized; (?) based on predictions.
| Name | Gene ID | Subcellular Localization | Main Substrate | Main Product | ||||
|---|---|---|---|---|---|---|---|---|
| Acyl Linked to: | Carbon Number | Presence of Double Bonds | Position and Configuration of the Introduced Double Bond | Overall Structure | Overall Structure | |||
| PAD/SAD | Phatraft_9316 | Chloroplast stroma | ACP | 16 and 18 | 0 | Δ9/cis | 16:0-ACP | 16:1Δ9-ACP |
| ADS | Phatr_28797 | Endomembrane system | CoA | 18? | 0 | Δ9/cis? | 18:0-CoA? | 18:1Δ9-CoA? |
| FAD2 (*) | Phatr_25769 | ER | Phospholipid/ Betaine lipid? | 18 | 1 | Δ12 (or ω6)/cis | 18:1Δ9-PL (-BL?) | 18:2Δ9,12-PL (-BL?) |
| ERΔ6FAD (*) | Phatr_2948 | ER | Phospholipid/ Betaine lipid? | 18 | 2 | Δ6/cis | 18:2Δ9,12-PL (-BL?) | 18:3Δ6,9,12-PL (-BL?) |
| ERω3FAD (?) | ? | ER | Phospholipid/ Betaine lipid? | 18 | 3 | Δ15 (or ω3)/cis | 18:3Δ6,9,12-PL (-BL?) | 18:4Δ6,9,12,15-PL (-BL?) |
| ERΔ5FAD.1 (*) ERΔ5FAD.2 (?) | Phatr_46830 Phatr_22459 | ER | Phospholipid/ Betaine lipid? | 20 | 4 | Δ5/cis | 20:4Δ8,11,14,17-PL (-BL?) | 20:5Δ5,8,11,14,17-PL (-BL?) |
| ERΔ4FAD | Phatr_22510? | ER | Phospholipid/ Betaine lipid? | 22 | 5 | Δ4/cis | 22:5Δ7,10,13,16,19-PL (-BL?) | 22:6Δ4,7,10,13,16,19-PL (-BL?) |
| FAD6 (*) | Phatr_48423 | Chloroplast membranes | sn1/sn2-MGDG/DGDG + SQDG | 16 | 1 | Δ12/cis | 16:1Δ9-sn2-MGDG/DGDG/SQDG | 16:2Δ9,12-sn1/sn2-MGDG/DGDG/SQDG |
| PlastidΔ6FAD (?) | Phatr_50443 | Chloroplast membranes | sn2-MGDG | 16 | 2 | Δ6/cis | 16:2Δ9,12-sn2-MGDG | 16:3Δ6,9,12-sn2-MGDG |
| Plastidω3FAD/FAD7 (?) | Phatr_41570 | Chloroplast membranes | sn2-MGDG? | 16 | 3 | ω3/cis | 16:3Δ6,9,12-sn2-MGDG | 16:4Δ6,9,12,15-sn2-MGDG |
| FAD4 | Phatr_41301 | Chloroplast membranes | sn2-PG | 16 | 0 | Δ3/trans | 16:0-sn2-PG | 16:1Δ3trans-sn2-PG |
On page 1330, line 9, “18:3 and 14” should be “18:3 and 18:4”. Line 29, in the title of Section 2.4.10, “The (18:2Δ9,12 → 18:3Δ6,9,12 and 18:3Δ9,12,15 → 18:3Δ6,9,12,15)-Desaturation” should be “The (18:2Δ9,12 → 18:3Δ6,9,12 and 18:3Δ9,12,15 → 18:4Δ6,9,12,15)-Desaturation”. On page 1331, line 6, in the title of Section 2.4.11, “The Question of the (18:2Δ9,12 → 18:3Δ6,9,15 and 18:3Δ6,9,12 → 18:4Δ6,9,12,15)-Desaturation” should be “The Question of the (18:2Δ9,12 → 18:3Δ9,12,15 and 18:3Δ6,9,12 → 18:4Δ6,9,12,15)-Desaturation”. On page 1332, line 1, “E Δ6FAD” should be “ERΔ6FAD”. On page 1335, lines 25–26, in the Conclusions Section, “ERD6FAD, and ERD5FAD” should be “ERΔ6FAD, and ERΔ5FAD”.
These changes have no material impact on the conclusions of our paper. We apologize to our readers for any inconvenience caused.
Reference
- Dolch, L.J.; Maréchal, E. Inventory of fatty acid desaturases in the pennate diatom Phaeodactylum tricornutum. Mar. Drugs 2015, 13, 1317–1339. [Google Scholar] [CrossRef] [PubMed]
© 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
Share and Cite
MDPI and ACS Style
Dolch, L.-J.; Maréchal, E. Dolch, L.-J. and Maréchal, E. Inventory of Fatty Acid Desaturases in the Pennate Diatom Phaeodactylum tricornutum. Mar. Drugs 2015, 13, 1317–1339. Mar. Drugs 2015, 13, 5732-5735. https://doi.org/10.3390/md13095732
AMA Style
Dolch L-J, Maréchal E. Dolch, L.-J. and Maréchal, E. Inventory of Fatty Acid Desaturases in the Pennate Diatom Phaeodactylum tricornutum. Mar. Drugs 2015, 13, 1317–1339. Marine Drugs. 2015; 13(9):5732-5735. https://doi.org/10.3390/md13095732
Chicago/Turabian StyleDolch, Lina-Juana, and Eric Maréchal. 2015. "Dolch, L.-J. and Maréchal, E. Inventory of Fatty Acid Desaturases in the Pennate Diatom Phaeodactylum tricornutum. Mar. Drugs 2015, 13, 1317–1339" Marine Drugs 13, no. 9: 5732-5735. https://doi.org/10.3390/md13095732
