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Mar. Drugs 2015, 13(9), 5736-5749;

Development and Application of a Novel SPE-Method for Bioassay-Guided Fractionation of Marine Extracts

Bio-Organic Chemistry Unit, Institute of Biomolecular Chemistry-CNR, Via Campi Flegrei 34, Pozzuoli, Naples 80078, Italy
Marine Biotechnology Laboratory, Integrative Marine Ecology Department, Stazione Zoologica Anton Dohrn, Villa Comunale, Naples 80121, Italy
GSK Vaccines s.r.l., via Fiorentina 1, Siena 53100, Italy
Author to whom correspondence should be addressed.
Academic Editor: Orazio Taglialatela-Scafati
Received: 16 June 2015 / Revised: 15 August 2015 / Accepted: 28 August 2015 / Published: 11 September 2015
(This article belongs to the Special Issue Marine Secondary Metabolites)
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The biological diversity of marine habitats is a unique source of chemical compounds with potential use as pharmaceuticals, cosmetics and dietary supplements. However, biological screening and chemical analysis of marine extracts pose specific technical constraints and require adequate sample preparation. Here we report an improved method on Solid Phase Extraction (SPE) to fractionate organic extracts containing high concentration of salt that hampers the recovery of secondary metabolites. The procedure uses a water suspension to load the extracts on a poly(styrene-divynylbenzene)-based support and a stepwise organic solvent elution to effectively desalt and fractionate the organic components. The novel protocol has been tested on MeOH-soluble material from three model organisms (Reniera sarai, Dendrilla membranosa and Amphidinium carterae) and was validated on a small panel of 47 marine samples, including sponges and protists, within discovery programs for identification of immuno-stimulatory and anti-infective natural products. View Full-Text
Keywords: drug discovery; bioactive marine natural products; SPE fractionation methods drug discovery; bioactive marine natural products; SPE fractionation methods

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Cutignano, A.; Nuzzo, G.; Ianora, A.; Luongo, E.; Romano, G.; Gallo, C.; Sansone, C.; Aprea, S.; Mancini, F.; D'Oro, U.; Fontana, A. Development and Application of a Novel SPE-Method for Bioassay-Guided Fractionation of Marine Extracts. Mar. Drugs 2015, 13, 5736-5749.

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