Esters of the Marine-Derived Triterpene Sipholenol A Reverse P-GP-Mediated Drug Resistance
AbstractOur previous studies showed that several sipholane triterpenes, sipholenol A, sipholenone E, sipholenol L and siphonellinol D, have potent reversal effect for multidrug resistance (MDR) in cancer cells that overexpressed P-glycoprotein (P-gp/ABCB1). Through comparison of cytotoxicity towards sensitive and multi-drug resistant cell lines, we identified that the semisynthetic esters sipholenol A-4-O-acetate and sipholenol A-4-O-isonicotinate potently reversed P-gp-mediated MDR but had no effect on MRP1/ABCC1 and BCRP/ABCG2-mediated MDR. The results from [3H]-paclitaxel accumulation and efflux studies suggested that these two triterpenoids were able to increase the intracellular accumulation of paclitaxel by inhibiting its active efflux. In addition, western blot analysis revealed that these two compounds did not alter the expression levels of P-gp when treated up to 72 h. These sipholenol derivatives also stimulated the ATPase activity of P-gp membranes, which suggested that they might be substrates of P-gp. Moreover, in silico molecular docking studies revealed the virtual binding modes of these two compounds into human homology model of P-gp. In conclusion, sipholenol A-4-O-acetate and sipholenol A-4-O-isonicotinate efficiently inhibit the P-gp and may represent potential reversal agents for the treatment of multidrug resistant cancers. View Full-Text
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Zhang, Y.; Zhang, Y.-K.; Wang, Y.-J.; Vispute, S.G.; Jain, S.; Chen, Y.; Li, J.; Youssef, D.T.A.; Sayed, K.A.E.; Chen, Z.-S. Esters of the Marine-Derived Triterpene Sipholenol A Reverse P-GP-Mediated Drug Resistance. Mar. Drugs 2015, 13, 2267-2286.
Zhang Y, Zhang Y-K, Wang Y-J, Vispute SG, Jain S, Chen Y, Li J, Youssef DTA, Sayed KAE, Chen Z-S. Esters of the Marine-Derived Triterpene Sipholenol A Reverse P-GP-Mediated Drug Resistance. Marine Drugs. 2015; 13(4):2267-2286.Chicago/Turabian Style
Zhang, Yongchao; Zhang, Yun-Kai; Wang, Yi-Jun; Vispute, Saurabh G.; Jain, Sandeep; Chen, Yangmin; Li, Jessalyn; Youssef, Diaa T.A.; Sayed, Khalid A.E.; Chen, Zhe-Sheng. 2015. "Esters of the Marine-Derived Triterpene Sipholenol A Reverse P-GP-Mediated Drug Resistance." Mar. Drugs 13, no. 4: 2267-2286.