Photo-Oxidative Stress-Driven Mutagenesis and Adaptive Evolution on the Marine Diatom Phaeodactylum tricornutum for Enhanced Carotenoid Accumulation
by
Zhiqian Yi 1,3,
Maonian Xu 4,
Manuela Magnusdottir 1,
Yuetuan Zhang 1,5,
Sigurdur Brynjolfsson 1 and
Weiqi Fu 1,2,*
Zhiqian Yi 1,3,
Maonian Xu 4,
Manuela Magnusdottir 1,
Yuetuan Zhang 1,5,
Sigurdur Brynjolfsson 1 and
Weiqi Fu 1,2,*
1
Center for Systems Biology and Faculty of Industrial Engineering, Mechanical Engineering and Computer Science, School of Engineering and Natural Sciences, University of Iceland, Reykjavík 101, Iceland
2
Division of Science and Math, New York University Abu Dhabi, and Center for Genomics and Systems Biology (CGSB), New York University Abu Dhabi Institute, Abu Dhabi 129188, UAE
3
Biomedical Center and Department of Life and Environmental Sciences, University of Iceland, Reykjavík 101, Iceland
4
Faculty of Pharmaceutical Sciences, University of Iceland, Hagi, Hofsvallagata 53, Reykjavik IS-107, Iceland
5
Department of Food and Environmental Sciences, Division of Food Chemistry, University of Helsinki, Helsinki FI-00014, Finland
*
Author to whom correspondence should be addressed.
Academic Editor: Véronique Martin-Jézéquel
Mar. Drugs 2015, 13(10), 6138-6151; https://doi.org/10.3390/md13106138
Received: 19 August 2015 / Revised: 12 September 2015 / Accepted: 15 September 2015 / Published: 29 September 2015
(This article belongs to the Special Issue Marine Carotenoids and Oxidative Stress)
Marine diatoms have recently gained much attention as they are expected to be a promising resource for sustainable production of bioactive compounds such as carotenoids and biofuels as a future clean energy solution. To develop photosynthetic cell factories, it is important to improve diatoms for value-added products. In this study, we utilized UVC radiation to induce mutations in the marine diatom Phaeodactylum tricornutum and screened strains with enhanced accumulation of neutral lipids and carotenoids. Adaptive laboratory evolution (ALE) was also used in parallel to develop altered phenotypic and biological functions in P. tricornutum and it was reported for the first time that ALE was successfully applied on diatoms for the enhancement of growth performance and productivity of value-added carotenoids to date. Liquid chromatography-mass spectrometry (LC-MS) was utilized to study the composition of major pigments in the wild type P. tricornutum, UV mutants and ALE strains. UVC radiated strains exhibited higher accumulation of fucoxanthin as well as neutral lipids compared to their wild type counterpart. In addition to UV mutagenesis, P. tricornutum strains developed by ALE also yielded enhanced biomass production and fucoxanthin accumulation under combined red and blue light. In short, both UV mutagenesis and ALE appeared as an effective approach to developing desired phenotypes in the marine diatoms via electromagnetic radiation-induced oxidative stress.
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Keywords:
Phaeodactylum tricornutum; UV mutagenesis; adaptive laboratory evolution (ALE); fucoxanthin; neutral lipids
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MDPI and ACS Style
Yi, Z.; Xu, M.; Magnusdottir, M.; Zhang, Y.; Brynjolfsson, S.; Fu, W. Photo-Oxidative Stress-Driven Mutagenesis and Adaptive Evolution on the Marine Diatom Phaeodactylum tricornutum for Enhanced Carotenoid Accumulation. Mar. Drugs 2015, 13, 6138-6151.
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