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Open AccessArticle

Squid Pen Chitin Chitooligomers as Food Colorants Absorbers

Life Science Development Center, Tamkang University, No. 151, Yingchuan Rd., Tamsui, New Taipei City 25137, Taiwan
Department of Chemistry, Tamkang University, New Taipei City 25137, Taiwan
Institute of Biotechnology & Environment, Tay Nguyen University, Buon Ma Thuot 63000, Vietnam
Author to whom correspondence should be addressed.
Academic Editors: David Harding and Hitoshi Sashiwa
Mar. Drugs 2015, 13(1), 681-696;
Received: 28 August 2014 / Accepted: 9 January 2015 / Published: 20 January 2015
(This article belongs to the Special Issue Advances in Marine Chitin and Chitosan)
One of the most promising applications of chitosanase is the conversion of chitinous biowaste into bioactive chitooligomers (COS). TKU033 chitosanase was induced from squid pen powder (SPP)-containing Bacillus cereus TKU033 medium and purified by ammonium sulfate precipitation and column chromatography. The enzyme was relatively more thermostable in the presence of the substrate and had an activity of 93% at 50 °C in a pH 5 buffer solution for 60 min. Furthermore, the enzyme used for the COS preparation was also studied. The enzyme products revealed various mixtures of COS that with different degrees of polymerization (DP), ranging from three to nine. In the culture medium, the fermented SPP was recovered, and it displayed a better adsorption rate (up to 96%) for the disperse dyes than the water-soluble food colorants, Allura Red AC (R40) and Tartrazne (Y4). Fourier transform-infrared spectroscopic (FT-IR) analysis proved that the adsorption of the dyes onto fermented SPP was a physical adsorption. Results also showed that fermented SPP was a favorable adsorber and could be employed as low-cost alternative for dye removal in wastewater treatment. View Full-Text
Keywords: chitosanase; squid pen; Bacillus cereus; chitooligomers; biosorbent chitosanase; squid pen; Bacillus cereus; chitooligomers; biosorbent
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Liang, T.-W.; Huang, C.-T.; Dzung, N.A.; Wang, S.-L. Squid Pen Chitin Chitooligomers as Food Colorants Absorbers. Mar. Drugs 2015, 13, 681-696.

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