Cracking the Cytotoxicity Code: Apoptotic Induction of 10-Acetylirciformonin B is Mediated through ROS Generation and Mitochondrial Dysfunction
Abstract
:1. Introduction
2. Results
2.1. The Apoptotic Induction Effect of 10AB in HL 60 Cells
2.2. The Effect of 10AB on Topoisomerase IIα Activity
2.3. The Relationship between 10AB-Induced Apoptosis in HL 60 Cells and the Disruption in Mitochondrial Membrane Potential as well as Mitochondrial Metabolism-Related Proteins
2.4. The Relationship between 10AB-Induced Apoptosis and ROS Generation
3. Discussion
4. Experimental Section
4.1. Bioassays Materials
4.2. Preparation of 10-Acetylirciformonin B (10AB) Stock Solution
4.3. MTT Proliferation Assay
4.4. Annexin V/PI Apoptosis Assay
4.5. Determination of ROS Generation, and MMP Disruption
4.6. Assay of Topoisomerase II Catalytic Inhibitors and Poisons
4.7. Co-Immunoprecipitaion and Western Blotting
4.8. Immunofluorescence Analysis
4.9. Statistics
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Shih, H.-C.; El-Shazly, M.; Juan, Y.-S.; Chang, C.-Y.; Su, J.-H.; Chen, Y.-C.; Shih, S.-P.; Chen, H.-M.; Wu, Y.-C.; Lu, M.-C. Cracking the Cytotoxicity Code: Apoptotic Induction of 10-Acetylirciformonin B is Mediated through ROS Generation and Mitochondrial Dysfunction. Mar. Drugs 2014, 12, 3072-3090. https://doi.org/10.3390/md12053072
Shih H-C, El-Shazly M, Juan Y-S, Chang C-Y, Su J-H, Chen Y-C, Shih S-P, Chen H-M, Wu Y-C, Lu M-C. Cracking the Cytotoxicity Code: Apoptotic Induction of 10-Acetylirciformonin B is Mediated through ROS Generation and Mitochondrial Dysfunction. Marine Drugs. 2014; 12(5):3072-3090. https://doi.org/10.3390/md12053072
Chicago/Turabian StyleShih, Huei-Chuan, Mohamed El-Shazly, Yung-Shun Juan, Chao-Yuan Chang, Jui-Hsin Su, Yu-Cheng Chen, Shou-Ping Shih, Huei-Mei Chen, Yang-Chang Wu, and Mei-Chin Lu. 2014. "Cracking the Cytotoxicity Code: Apoptotic Induction of 10-Acetylirciformonin B is Mediated through ROS Generation and Mitochondrial Dysfunction" Marine Drugs 12, no. 5: 3072-3090. https://doi.org/10.3390/md12053072