Evaluation of the Age- and Sex-Related Changes of the Osteogenic Differentiation Potentials of Healthy Bone Marrow-Derived Mesenchymal Stem Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bone Marrow-Derived Mesenchymal Stem Cells
2.2. Cellular Morphology and Determination of Cell Viability
2.3. Immunofluorescence
2.4. Secretion of Human Vascular Endothelial Growth Factor from the BMSCs
2.5. Total RNA Extraction and Quantification Using a Real-Time Polymerase Chain Reaction
2.6. Western Blotting Analysis
2.7. Evaluation of the Adenosine 5-Triphosphate Assay
2.8. Alkaline Phosphatase Activity
2.9. Alizarin Red S Staining
2.10. Statistical Analysis
3. Results
3.1. Cellular Morphology and Cell Viability
3.2. Immunofluorescence and Secretion of Human Vascular Endothelial Growth Factor from the BMSCs
3.3. Validation of mRNA Expression by qPCR
3.4. Western Blot
3.5. Evaluation of the Adenosine 5-Triphosphate Assay
3.6. Alkaline Phosphatase Activity
3.7. Osteogenic Differentiation Potential by Alizarin Red S Staining
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Lee, H.-J.; Lee, H.; Na, C.-B.; Song, I.-S.; Ryu, J.-J.; Park, J.-B. Evaluation of the Age- and Sex-Related Changes of the Osteogenic Differentiation Potentials of Healthy Bone Marrow-Derived Mesenchymal Stem Cells. Medicina 2021, 57, 520. https://doi.org/10.3390/medicina57060520
Lee H-J, Lee H, Na C-B, Song I-S, Ryu J-J, Park J-B. Evaluation of the Age- and Sex-Related Changes of the Osteogenic Differentiation Potentials of Healthy Bone Marrow-Derived Mesenchymal Stem Cells. Medicina. 2021; 57(6):520. https://doi.org/10.3390/medicina57060520
Chicago/Turabian StyleLee, Hyun-Jin, Hyuna Lee, Chae-Bin Na, In-Seok Song, Jae-Jun Ryu, and Jun-Beom Park. 2021. "Evaluation of the Age- and Sex-Related Changes of the Osteogenic Differentiation Potentials of Healthy Bone Marrow-Derived Mesenchymal Stem Cells" Medicina 57, no. 6: 520. https://doi.org/10.3390/medicina57060520