Celiac-Related Autoantibodies and IL-17A in Bulgarian Patients with Dermatitis Herpetiformis: A Cross-Sectional Study
Abstract
:1. Introduction
2. Material and Methods
2.1. Serum Samples
2.2. Immune Serology Testing
2.2.1. Immunoenzyme Testing
- anti-tTG antibodies (Anti-Tissue Transglutaminase Screen IgA + IgG, Orgentec Diagnostika GmbH, cut-off value > 10 U/mL);
- anti-DGP antibodies (Quanta Lite Celiac DGP Screen IgA + IgG, Inova Diagnostics, Inc., San Diego, USA, cut-off > 15 U/mL);
- AAA (Quanta Lite F-Actin IgA ELISA, Inova Diagnostics, Inc., San Diego, USA, cut-off > 20 U/mL);
- IL-17A (Human IL-17A ELISA kit, Diaclone, GenProbe, France, sensitivity < 2.3 pg/mL).
2.2.2. Immunoblot Testing
2.3. Statistical Analysis
3. Results
3.1. Serum Levels of the Celiac Disease-Related Autoantibodies and the Pro-Inflammatory Cytokine IL-17A
3.2. Performance Characteristics of the Celiac-Related Antibodies Tested in DH Patients
3.3. ROC Curve Analysis of the Celiac Disease-Related Antibodies and IL-17A in DH Patients
3.4. Correlation between Tests
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Anti-tTG IgA + IgG (ELISA) | Anti-DGP IgA + IgG (ELISA) | AAA IgG (ELISA) | Anti-tTG IgG (Line Blot) | AGA IgG (Line Blot) | |
---|---|---|---|---|---|
Sensitivity | 42.3% | 46.4% | 34.7% | 46% | 50% |
Specificity | 100% | 95% | 100% | 100% | 100% |
PPV * | 100% | 90.9% | 100% | 100% | 100% |
NPV ** | 57% | 57.1% | 54.1% | 59% | 60% |
Anti-tTG (ELISA) | Anti-DGP (ELISA) | AAA (ELISA) | IL-17A (ELISA) | Anti-tTG (Line Blot) | AGA (Line Blot) | |
---|---|---|---|---|---|---|
Anti-tTG (ELISA) | r = 0.894 p < 0.001 | r = 0.863 p = 0.001 | r = 0.938 p < 0.001 | r = 0.520 p = 0.003 | r = 0.507 p = 0.076 | |
anti-DGP (ELISA) | r = 0.502 p = 0.009 | r = 0.452 p = 0.031 | r = 0.532 p = 0.001 | r = 0.346 p = 0.038 | ||
AAA (ELISA) | r = 0.692 p < 0.001 | r = 0.112 p = 0.500 | r = 0.221 p = 0.186 | |||
IL-17A(ELISA) | r = 0.079 p = 0.676 | r = −0.222 p = 0.238 | ||||
Anti-tTG (Line blot) | r = 0.678 p < 0.001 | |||||
AGA (Line blot) |
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Velikova, T.; Shahid, M.; Ivanova-Todorova, E.; Drenovska, K.; Tumangelova-Yuzeir, K.; Altankova, I.; Vassileva, S. Celiac-Related Autoantibodies and IL-17A in Bulgarian Patients with Dermatitis Herpetiformis: A Cross-Sectional Study. Medicina 2019, 55, 136. https://doi.org/10.3390/medicina55050136
Velikova T, Shahid M, Ivanova-Todorova E, Drenovska K, Tumangelova-Yuzeir K, Altankova I, Vassileva S. Celiac-Related Autoantibodies and IL-17A in Bulgarian Patients with Dermatitis Herpetiformis: A Cross-Sectional Study. Medicina. 2019; 55(5):136. https://doi.org/10.3390/medicina55050136
Chicago/Turabian StyleVelikova, Tsvetelina, Martin Shahid, Ekaterina Ivanova-Todorova, Kossara Drenovska, Kalina Tumangelova-Yuzeir, Iskra Altankova, and Snejina Vassileva. 2019. "Celiac-Related Autoantibodies and IL-17A in Bulgarian Patients with Dermatitis Herpetiformis: A Cross-Sectional Study" Medicina 55, no. 5: 136. https://doi.org/10.3390/medicina55050136