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Article

Magnolia kobus Extract Inhibits Periodontitis-Inducing Mediators in Porphyromonas gingivalis Lipopolysaccharide-Activated RAW 264.7 Cells

by
Hae-Jin Lee
,
So-Jung Lee
,
Sung-Kwon Lee
,
Bong-Keun Choi
and
Dong-Ryung Lee
*
Research Institute, NUON Co., Ltd., Seongnam 13201, Republic of Korea
*
Author to whom correspondence should be addressed.
Curr. Issues Mol. Biol. 2023, 45(1), 538-554; https://doi.org/10.3390/cimb45010036
Submission received: 21 December 2022 / Revised: 5 January 2023 / Accepted: 6 January 2023 / Published: 6 January 2023
(This article belongs to the Special Issue Advances in Research on Molecular Oral Microorganisms)
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Abstract

Periodontitis, a disease caused by inflammation of oral bacteria, contributes to the loss of alveolar bone and destruction of connective tissues. Porphyromonas gingivalis, a Gram-negative bacterium, is known to possess important pathogenic factors for periodontal disease. In this study, we investigated the anti-periodontitis effects of Magnolia kobus extract (MKE) and magnolin as a component of Magnolia kobus (MK) in murine macrophage RAW 264.7 cells stimulated with Porphyromonas gingivalis lipopolysaccharide (LPS). Effects of MKE and magnolin on the mechanism of RAW 264.7 cellular inflammation were determined by analyzing nitric oxide (NO) production and Western blot protein expression (n = 3). MKE/magnolin inhibited NO production without affecting cell survival. MKE/magnolin treatment inhibited LPS-induced pro-inflammatory cytokines, expression levels of matrix metalloproteinases (MMPs such as MMP-1, 3, 8, 9, and 13), and protein levels of inflammatory mediators (such as TNF-α, IL-1β, and mPGES-1). MKE/magnolin also suppressed NF-κB activation by inhibiting the TLR4 signaling pathway. These findings suggest that MKE has a therapeutic effect on inflammatory periodontal disease caused by oral bacterium P. gingivalis and that magnolin is a major functional component in the anti-inflammatory effect of MKE.
Keywords: Magnolia kobus; magnolin; RAW 264.7; periodontitis; Porphyromonas gingivalis; inflammation; MMPs; NF-κB; MAPK Magnolia kobus; magnolin; RAW 264.7; periodontitis; Porphyromonas gingivalis; inflammation; MMPs; NF-κB; MAPK

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MDPI and ACS Style

Lee, H.-J.; Lee, S.-J.; Lee, S.-K.; Choi, B.-K.; Lee, D.-R. Magnolia kobus Extract Inhibits Periodontitis-Inducing Mediators in Porphyromonas gingivalis Lipopolysaccharide-Activated RAW 264.7 Cells. Curr. Issues Mol. Biol. 2023, 45, 538-554. https://doi.org/10.3390/cimb45010036

AMA Style

Lee H-J, Lee S-J, Lee S-K, Choi B-K, Lee D-R. Magnolia kobus Extract Inhibits Periodontitis-Inducing Mediators in Porphyromonas gingivalis Lipopolysaccharide-Activated RAW 264.7 Cells. Current Issues in Molecular Biology. 2023; 45(1):538-554. https://doi.org/10.3390/cimb45010036

Chicago/Turabian Style

Lee, Hae-Jin, So-Jung Lee, Sung-Kwon Lee, Bong-Keun Choi, and Dong-Ryung Lee. 2023. "Magnolia kobus Extract Inhibits Periodontitis-Inducing Mediators in Porphyromonas gingivalis Lipopolysaccharide-Activated RAW 264.7 Cells" Current Issues in Molecular Biology 45, no. 1: 538-554. https://doi.org/10.3390/cimb45010036

APA Style

Lee, H.-J., Lee, S.-J., Lee, S.-K., Choi, B.-K., & Lee, D.-R. (2023). Magnolia kobus Extract Inhibits Periodontitis-Inducing Mediators in Porphyromonas gingivalis Lipopolysaccharide-Activated RAW 264.7 Cells. Current Issues in Molecular Biology, 45(1), 538-554. https://doi.org/10.3390/cimb45010036

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