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Bacterial Sub-Species Typing Using Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry: What Is Promising?

University of Queensland Centre for Clinical Research, The University of Queensland, Herston, Brisbane, QLD 4029, Australia
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Academic Editor: Shubham Dutta
Curr. Issues Mol. Biol. 2021, 43(2), 749-757; https://doi.org/10.3390/cimb43020054
Received: 16 June 2021 / Revised: 7 July 2021 / Accepted: 16 July 2021 / Published: 20 July 2021
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology Section)
Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) is routinely used for bacterial identification. It would be highly beneficial to also be able to use the technology as a fast way to detect clinically relevant clones of bacterial species. However, studies to this aim have often had limited success. The methods used for data acquisition, processing and data interpretation are highly diverse amongst studies on MALDI-TOF MS sub-species typing. In addition to this, feasibility may depend on the bacterial species and strains investigated, making it difficult to determine what methods may or may not work. In our paper, we have reviewed recent research on MALDI-TOF MS typing of bacterial strains. Although we found a lot of variation amongst the methods used, there were approaches shared by multiple research groups. Multiple spectra of the same isolate were often combined before further analysis for strain distinction. Many groups used a protein extraction step to increase resolution in their MALDI-TOF MS results. Peaks at a high mass range were often excluded for data interpretation. Three groups have found ways to determine feasibility of MALDI-TOF MS typing for their set of strains at an early stage of their project. View Full-Text
Keywords: MALDI-TOF MS typing; MALDI-TOF MS; bacterial sub-species typing; strain typing; bacterial strain identification MALDI-TOF MS typing; MALDI-TOF MS; bacterial sub-species typing; strain typing; bacterial strain identification
MDPI and ACS Style

Huber, C.A.; Reed, S.J.; Paterson, D.L. Bacterial Sub-Species Typing Using Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry: What Is Promising? Curr. Issues Mol. Biol. 2021, 43, 749-757. https://doi.org/10.3390/cimb43020054

AMA Style

Huber CA, Reed SJ, Paterson DL. Bacterial Sub-Species Typing Using Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry: What Is Promising? Current Issues in Molecular Biology. 2021; 43(2):749-757. https://doi.org/10.3390/cimb43020054

Chicago/Turabian Style

Huber, Charlotte A., Sarah J. Reed, and David L. Paterson 2021. "Bacterial Sub-Species Typing Using Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry: What Is Promising?" Current Issues in Molecular Biology 43, no. 2: 749-757. https://doi.org/10.3390/cimb43020054

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