Pretreatment of Garlic Oil Extracts Hampers Epithelial Damage in Cell Culture Model of Peptic Ulcer Disease
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Sodium Taurocholate-Induced Damage to AGS Cells
2.3. Measurement of the Gastroprotective Effect of Garlic Extracts (GE) in a Cell Culture Model of Peptic Ulcer Disease
2.4. Measurement of Cellular Glutathione (GSH) Concentration
2.5. Measurement of Prostaglandin E2 (PGE2) Concentration
2.6. Total RNA Isolation and Reverse Transcription Polymerase Chain Reaction (RT-PCR) Analysis
2.7. Visualization of the F-Actin Cytoskelet with Rhodamine Phalloidin Stain
2.8. Statistical Analyses
3. Results
3.1. Establishment of the Cell Culture Model of Peptic Ulcer Disease and Assessment of the Toxic Effect of Sodium Taurocholate (NaT)
3.2. Gastroprotective Effect of Garlic Extracts (GE) in a Cell Culture Model of Peptic Ulcer Disease
3.3. Measurement of Cellular Glutathione (GSH) Concentration in a Cell Culture Model of Peptic Ulcer Disease
3.4. Measurement of Prostaglandin E2 (PGE2) Concentration in a Cell Culture Model of Peptic Ulcer Disease
3.5. Visualization and Quantification of the F-Actin Cytoskeleton with Rhodamine Phalloidin Stain in a Cell Culture Model of Peptic Ulcer Disease
3.6. Expression of ABCG2 and NFκB2 in a Cell Culture Model of Peptic Ulcer Disease
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
ABCG2 | ATP-binding cassette, sub-family G, member 2 |
AGS | Adenocarcinoma epithelial gastric cell line |
BSA | Bovine serum albumin |
CCl | Tetrachloride |
DAPI | 4,6-diamidino-2-phenylindole |
DATS | Diallyl trisulfide |
FBS | Fetal bovine serum |
GE | Garlic oil extracts |
GI | Gastrointestinal |
GSH | Glutathione |
H. pylori | Helicobacter pylori |
IFN-γ | Interferon gamma |
IL-17 | Interleukin 17 |
IL-1 | Interleukin 1 |
IL-6 | Interleukin 6 |
LPZ | Lansoprazole |
MIP-1 | Macrophage Inflammatory Protein 2 |
MTT | 3-(4, 5-dimethyl-2-thiazolyl) -2, 5-diphenyl-2H- tetrazolium bromide |
NaT | Sodium taurocholate |
NFκB2 | Nuclear factor kappa B subunit 2 |
NSAID | Non-steroidal anti-inflammatory drugs |
PBS | Phosphate buffered saline |
PG | Prostaglandin |
PGE2 | Prostaglandin E2 |
PPI | Proton pump inhibitors |
PUD | Peptic ulcer disease |
RANTES | Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted |
ROS | Reactive oxygen species |
SAC | S-allyl-cysteine |
SOD | Superoxide dismutases |
TNF-α | Tumor necrosis factor alpha |
TGF-β1 | Transforming growth factor beta 1 |
tTG | Transglutaminase |
TIMP-1 | Tissue metalloproteinase inhibitor |
SAC | S-allyl-cysteine |
α-SMA | α-smooth muscle actin |
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Kuna, L.; Zjalic, M.; Kizivat, T.; Roguljic, H.; Nincevic, V.; Omanovic Kolaric, T.; Wu, C.H.; Vcev, A.; Smolic, M.; Smolic, R. Pretreatment of Garlic Oil Extracts Hampers Epithelial Damage in Cell Culture Model of Peptic Ulcer Disease. Medicina 2022, 58, 91. https://doi.org/10.3390/medicina58010091
Kuna L, Zjalic M, Kizivat T, Roguljic H, Nincevic V, Omanovic Kolaric T, Wu CH, Vcev A, Smolic M, Smolic R. Pretreatment of Garlic Oil Extracts Hampers Epithelial Damage in Cell Culture Model of Peptic Ulcer Disease. Medicina. 2022; 58(1):91. https://doi.org/10.3390/medicina58010091
Chicago/Turabian StyleKuna, Lucija, Milorad Zjalic, Tomislav Kizivat, Hrvoje Roguljic, Vjera Nincevic, Tea Omanovic Kolaric, Catherine H. Wu, Aleksandar Vcev, Martina Smolic, and Robert Smolic. 2022. "Pretreatment of Garlic Oil Extracts Hampers Epithelial Damage in Cell Culture Model of Peptic Ulcer Disease" Medicina 58, no. 1: 91. https://doi.org/10.3390/medicina58010091