Next Article in Journal
Potential Use of Polyamidoamine Dendrimer Conjugates with Cyclodextrins as Novel Carriers for siRNA
Next Article in Special Issue
Selecting Molecular Recognition. What Can Existing Aptamers Tell Us about Their Inherent Recognition Capabilities and Modes of Interaction?
Previous Article in Journal
New Strategies to Develop Novel Pain Therapies: Addressing Thermoreceptors from Different Points of View
Previous Article in Special Issue
Coupling Aptamers to Short Interfering RNAs as Therapeutics
Open AccessArticle

Anti-HCV RNA Aptamers Targeting the Genomic cis-Acting Replication Element

Instituto de Parasitología y Biomedicina “López-Neyra”, IPBLN-CSIC, P.T. Ciencias de la Salud, Av. del Conocimiento s/n, Armilla, 18100 Granada, Spain
Author to whom correspondence should be addressed.
Pharmaceuticals 2012, 5(1), 49-60;
Received: 28 November 2011 / Revised: 15 December 2011 / Accepted: 22 December 2011 / Published: 28 December 2011
(This article belongs to the Special Issue Aptamer-Based Therapeutics)
Hepatitis C virus (HCV) replication is dependent on the existence of several highly conserved functional genomic RNA domains. The cis-acting replication element (CRE), located within the 3' end of the NS5B coding region of the HCV genome, has been shown essential for efficient viral replication. Its sequence and structural features determine its involvement in functional interactions with viral RNA-dependent RNA polymerase and distant RNA domains of the viral genome. This work reports the use of an in vitro selection strategy to select aptamer RNA molecules against the complete HCV-CRE. After six selection cycles, five potential target sites were identified within this domain. Inhibition assays using a sample of representative aptamers showed that the selected RNAs significantly inhibit the replication (>80%) of a subgenomic HCV replicon in Huh-7 cell cultures. These results highlight the potential of aptamer RNA molecules as therapeutic antiviral agents. View Full-Text
Keywords: aptamer selection; SELEX; RNA aptamers; anti-HCV aptamers; aptamer therapeutics aptamer selection; SELEX; RNA aptamers; anti-HCV aptamers; aptamer therapeutics
Show Figures

Figure 1

MDPI and ACS Style

Marton, S.; Berzal-Herranz, B.; Garmendia, E.; Cueto, F.J.; Berzal-Herranz, A. Anti-HCV RNA Aptamers Targeting the Genomic cis-Acting Replication Element. Pharmaceuticals 2012, 5, 49-60.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

Only visits after 24 November 2015 are recorded.
Back to TopTop