Serpentine Enhances Insulin Regulation of Blood Glucose through Insulin Receptor Signaling Pathway
Abstract
:1. Introduction
2. Results
2.1. Cytotoxicity of Serpentine and Affinity of Serpentine for Insulin Receptor
2.2. Effects of Serpentine on the Insulin Signaling Pathway Activated by Low-Dose Insulin
2.3. Effects of Serpentine on Blood Glucose and Hormone Levels in Mice with STZ/HFD-Induced Type 2 Diabetes Treated with Insulin
2.4. Effects of Serpentine and Insulin Treatment on the Insulin Signaling Pathway in Target Organs
2.5. Effects of Serpentine and Insulin Treatment on Genes Related to Gluconeogenesis, Glycogen Synthesis, and Fat Synthesis
2.6. Serpentine Promotes Glucose Uptake through the AMPK Signaling Pathway
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Treatments
4.2. Animals
4.3. Cell Viability Assay
4.4. Western Blot Analysis
4.5. Real-Time Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR)
4.6. Glucose Uptake Assay
4.7. SPR Analysis
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene Name | Forward Primer | Reverse Primer |
---|---|---|
β-Actin | 5′-GAGACCTTCAACACCCCAGC-3′ | 5′-ATGTCACGCACGATTTCCC-3′ |
Gys | 5′-ATCTTCTTCGTCTTCCGCATC-3′ | 5′-GACACTGAGCAGGGCTTTTCC-3′ |
G6pase | 5′-AAAAAGCCAACGTATGGATTCCG-3′ | 5′-CAGCAAGGTAGATCCGGGA-3′ |
Pepck | 5′-TTTGATGCCCAAGGCAACTT-3′ | 5′-ATCGATGCCTTCCCAGTAAA-3′ |
Fas | 5′-CTGGCATTCGTGATGGAGTC-3′ | 5′-TGTTTCCCCTGAGCCATGTA-3′ |
Acc1 | 5′-CGCTCGTCAGGTTCTTATTG-3′ | 5′-TTTCTGCAGGTTCTCAATGC-3′ |
Ugp2 | 5′-TAACCAAGGGCACTGTAGGGA-3′ | 5′-GGAGCTGCAATTAAAAGTTTCG-3′ |
Gsk3β | 5′-TCCATTCCTTTGGAATCTGC-3′ | 5′-CAATTCAGCCAACACACAGC-3′ |
α-Tubulin | 5′-CCACAAGTTTGATCTGTTGCAT-3′ | 5′-GCAGCAACTAGTATCCCTGTCC-3′ |
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Wang, Y.; Liu, G.; Liu, X.; Chen, M.; Zeng, Y.; Li, Y.; Wu, X.; Wang, X.; Sheng, J. Serpentine Enhances Insulin Regulation of Blood Glucose through Insulin Receptor Signaling Pathway. Pharmaceuticals 2023, 16, 16. https://doi.org/10.3390/ph16010016
Wang Y, Liu G, Liu X, Chen M, Zeng Y, Li Y, Wu X, Wang X, Sheng J. Serpentine Enhances Insulin Regulation of Blood Glucose through Insulin Receptor Signaling Pathway. Pharmaceuticals. 2023; 16(1):16. https://doi.org/10.3390/ph16010016
Chicago/Turabian StyleWang, Yinghao, Guanfu Liu, Xutao Liu, Minhua Chen, Yuping Zeng, Yuyan Li, Xiaoyun Wu, Xuanjun Wang, and Jun Sheng. 2023. "Serpentine Enhances Insulin Regulation of Blood Glucose through Insulin Receptor Signaling Pathway" Pharmaceuticals 16, no. 1: 16. https://doi.org/10.3390/ph16010016