Next Article in Journal
Zebrafish as a Screening Model to Study the Single and Joint Effects of Antibiotics
Previous Article in Journal
Glossogyne tenuifolia Extract Increases Nitric Oxide Production in Human Umbilical Vein Endothelial Cells
Article

Calcium Chelidonate: Semi-Synthesis, Crystallography, and Osteoinductive Activity In Vitro and In Vivo

1
Department of Pharmaceutical Analysis, Siberian State Medical University, 634050 Tomsk, Russia
2
Department of Morphology and General Pathology, Siberian State Medical University, 634050 Tomsk, Russia
3
Research School of Chemistry & Applied Biomedical Sciences, Tomsk Polytechnic University, 634050 Tomsk, Russia
4
Center of Spectral Investigations, Novosibirsk Institute of Organic Chemistry, Siberian Branch, 630090 Novosibirsk, Russia
5
Laboratory of Medicinal Chemistry, Novosibirsk Institute of Organic Chemistry, Siberian Branch, 630090 Novosibirsk, Russia
6
Center for Immunology and Cell Biotechnology, Immanuel Kant Baltic Federal University, 236041 Kaliningrad, Russia
*
Author to whom correspondence should be addressed.
Academic Editor: Félix Carvalho
Pharmaceuticals 2021, 14(6), 579; https://doi.org/10.3390/ph14060579
Received: 24 May 2021 / Revised: 11 June 2021 / Accepted: 14 June 2021 / Published: 17 June 2021
(This article belongs to the Section Pharmacology)
Calcium chelidonate [Ca(ChA)(H2O)3]n was obtained by semi-synthesis using natural chelidonic acid. The structure of the molecular complex was determined by X-ray diffraction analysis. The asymmetric unit of [Ca(ChA)(H2O)3]n includes chelidonic acid coordinated through three oxygen atoms, and three water ligands. The oxygen atoms of acid and oxygen atoms of water from each asymmetric unit are also coordinated to the calcium of another one, forming an infinite linear complex. Calcium geometry is close to the trigonal dodecahedron (D2d). The intra-complex hydrogen bonds additionally stabilize the linear species, which are parallel to the axis. In turn the linear species are packed into the 3D structure through mutual intercomplex hydrogen bonds. The osteogenic activity of the semi-synthetic CaChA was studied in vitro on 21-day hAMMSC culture and in vivo in mice using ectopic (subcutaneous) implantation of CaP-coated Ti plates saturated in vitro with syngeneic bone marrow. The enhanced extracellular matrix ECM mineralization in vitro and ectopic bone tissue formation in situ occurred while a water solution of calcium chelidonate at a dose of 10 mg/kg was used. The test substance promotes human adipose-derived multipotent mesenchymal stromal/stem cells (hAMMSCs), as well as mouse MSCs to differentiate into osteoblasts in vitro and in vivo, respectively. Calcium chelidonate is non-toxic and can stimulate osteoinductive processes. View Full-Text
Keywords: calcium chelidonate; semi-synthesis; X-ray diffraction analysis; osteogenic activity calcium chelidonate; semi-synthesis; X-ray diffraction analysis; osteogenic activity
Show Figures

Figure 1

MDPI and ACS Style

Avdeeva, E.; Porokhova, E.; Khlusov, I.; Rybalova, T.; Shults, E.; Litvinova, L.; Shupletsova, V.; Khaziakhmatova, O.; Sukhodolo, I.; Belousov, M. Calcium Chelidonate: Semi-Synthesis, Crystallography, and Osteoinductive Activity In Vitro and In Vivo. Pharmaceuticals 2021, 14, 579. https://doi.org/10.3390/ph14060579

AMA Style

Avdeeva E, Porokhova E, Khlusov I, Rybalova T, Shults E, Litvinova L, Shupletsova V, Khaziakhmatova O, Sukhodolo I, Belousov M. Calcium Chelidonate: Semi-Synthesis, Crystallography, and Osteoinductive Activity In Vitro and In Vivo. Pharmaceuticals. 2021; 14(6):579. https://doi.org/10.3390/ph14060579

Chicago/Turabian Style

Avdeeva, Elena, Ekaterina Porokhova, Igor Khlusov, Tatyana Rybalova, Elvira Shults, Larisa Litvinova, Valeria Shupletsova, Olga Khaziakhmatova, Irina Sukhodolo, and Mikhail Belousov. 2021. "Calcium Chelidonate: Semi-Synthesis, Crystallography, and Osteoinductive Activity In Vitro and In Vivo" Pharmaceuticals 14, no. 6: 579. https://doi.org/10.3390/ph14060579

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop