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Volume 23
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Review

Multi-Illumination Single-Holographic-Exposure Lensless Fresnel (MISHELF) Microscopy: Principles and Biomedical Applications

by
José Ángel Picazo-Bueno
1,2,*,
Martín Sanz
1,
Luis Granero
1,
Javier García
1 and
Vicente Micó
1,*
1
Department of Optics, Optometry and Vision Science, University of Valencia, 46100 Burjassot, Spain
2
Biomedical Technology Center of the Medical Faculty, University of Muenster, Mendelstr. 17, D-48149 Muenster, Germany
*
Authors to whom correspondence should be addressed.
Sensors 2023, 23(3), 1472; https://doi.org/10.3390/s23031472
Submission received: 17 December 2022 / Revised: 13 January 2023 / Accepted: 19 January 2023 / Published: 28 January 2023
(This article belongs to the Collection Biomedical Imaging and Sensing)
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Abstract

Lensless holographic microscopy (LHM) comes out as a promising label-free technique since it supplies high-quality imaging and adaptive magnification in a lens-free, compact and cost-effective way. Compact sizes and reduced prices of LHMs make them a perfect instrument for point-of-care diagnosis and increase their usability in limited-resource laboratories, remote areas, and poor countries. LHM can provide excellent intensity and phase imaging when the twin image is removed. In that sense, multi-illumination single-holographic-exposure lensless Fresnel (MISHELF) microscopy appears as a single-shot and phase-retrieved imaging technique employing multiple illumination/detection channels and a fast-iterative phase-retrieval algorithm. In this contribution, we review MISHELF microscopy through the description of the principles, the analysis of the performance, the presentation of the microscope prototypes and the inclusion of the main biomedical applications reported so far.
Keywords: multiplexed holography; digital holography; in-line holographic microscopy; Gabor holography; lensless microscopy; digital image processing; label-free imaging; biomedical imaging multiplexed holography; digital holography; in-line holographic microscopy; Gabor holography; lensless microscopy; digital image processing; label-free imaging; biomedical imaging

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MDPI and ACS Style

Picazo-Bueno, J.Á.; Sanz, M.; Granero, L.; García, J.; Micó, V. Multi-Illumination Single-Holographic-Exposure Lensless Fresnel (MISHELF) Microscopy: Principles and Biomedical Applications. Sensors 2023, 23, 1472. https://doi.org/10.3390/s23031472

AMA Style

Picazo-Bueno JÁ, Sanz M, Granero L, García J, Micó V. Multi-Illumination Single-Holographic-Exposure Lensless Fresnel (MISHELF) Microscopy: Principles and Biomedical Applications. Sensors. 2023; 23(3):1472. https://doi.org/10.3390/s23031472

Chicago/Turabian Style

Picazo-Bueno, José Ángel, Martín Sanz, Luis Granero, Javier García, and Vicente Micó. 2023. "Multi-Illumination Single-Holographic-Exposure Lensless Fresnel (MISHELF) Microscopy: Principles and Biomedical Applications" Sensors 23, no. 3: 1472. https://doi.org/10.3390/s23031472

APA Style

Picazo-Bueno, J. Á., Sanz, M., Granero, L., García, J., & Micó, V. (2023). Multi-Illumination Single-Holographic-Exposure Lensless Fresnel (MISHELF) Microscopy: Principles and Biomedical Applications. Sensors, 23(3), 1472. https://doi.org/10.3390/s23031472

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