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Article

Immobilized Antibodies on Mercaptophenylboronic Acid Monolayers for Dual-Strategy Detection of 20S Proteasome

1
National Institute of Materials Physics, Atomistilor 405A, 077125 Măgurele, Romania
2
Faculty of Physics, University of Bucharest, Atomistilor 405, 077125 Măgurele, Romania
*
Author to whom correspondence should be addressed.
Academic Editor: James F. Rusling
Sensors 2021, 21(8), 2702; https://doi.org/10.3390/s21082702
Received: 16 March 2021 / Revised: 5 April 2021 / Accepted: 8 April 2021 / Published: 12 April 2021
(This article belongs to the Special Issue Recent Advances in Immunosensors and Biosensors)
A dual strategy for the electrochemical detection for 20S proteasome (20S) is proposed, based on the oriented immobilization of a capture monoclonal antibody (Abβ) on a self-assembled monolayer of 4-mercaptophenylboronic acid (4-MPBA) on gold electrodes, which led to the Au/4-MPBA/Abβ immunosensor. The methodology comprises the correlation of 20S concentration with (i) its proteolytic activity toward the Z-LLE-AMC substrate, using the Au/4-MPBA/Abβ/20S, and (ii) the enzymatic activity of an alkaline phosphatase (AlkP) from the AlkP-labeled secondary antibody (Abcore-AlkP), which involves the conversion of aminophenylphosphate to the electroactive aminophenol using Au/4-MPBA/Abβ/20S/Abcore-AlkP. The step-by-step construction of the immunosensor and the interactions at its surface were evaluated by surface plasmon resonance and gravimetric analysis with quartz crystal microbalance, showing a high affinity between both antibodies and 20S. Morphological analysis by scanning electron microscopy demonstrated a pattern of parallel lines upon immobilization of Abβ on 4-MPBA and morphological changes to a well-organized granular structure upon binding of 20S. A voltametric and impedimetric characterization was performed after each step in the immunosensor construction. The two detection strategies were evaluated. It was shown that the immunosensor responds linearly with 20S concentration in the range between 5 and 100 µg mL−1, which corresponds to proteasome levels in serum in the case of diverse pathological situations, and LoD values of 1.4 and 0.2 µg mL−1 were calculated for the detection strategies. The immunosensor was applied to the detection of 20S in serum samples with recovery values ranging from 101 to 103%. View Full-Text
Keywords: electrochemistry; immunosensor; oriented antibodies; mercaptophenylboronic acid; self-assembled monolayers; 20S proteasome; enzymatic activity; proteolysis; labeled antibody; alkaline phosphatase electrochemistry; immunosensor; oriented antibodies; mercaptophenylboronic acid; self-assembled monolayers; 20S proteasome; enzymatic activity; proteolysis; labeled antibody; alkaline phosphatase
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MDPI and ACS Style

Barsan, M.M.; Sanz, C.G.; Onea, M.; Diculescu, V.C. Immobilized Antibodies on Mercaptophenylboronic Acid Monolayers for Dual-Strategy Detection of 20S Proteasome. Sensors 2021, 21, 2702. https://doi.org/10.3390/s21082702

AMA Style

Barsan MM, Sanz CG, Onea M, Diculescu VC. Immobilized Antibodies on Mercaptophenylboronic Acid Monolayers for Dual-Strategy Detection of 20S Proteasome. Sensors. 2021; 21(8):2702. https://doi.org/10.3390/s21082702

Chicago/Turabian Style

Barsan, Madalina M., Caroline G. Sanz, Melania Onea, and Victor C. Diculescu 2021. "Immobilized Antibodies on Mercaptophenylboronic Acid Monolayers for Dual-Strategy Detection of 20S Proteasome" Sensors 21, no. 8: 2702. https://doi.org/10.3390/s21082702

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