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Sensors
Volume 21
Issue 2
10.3390/s21020377
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Letter

Integrated, Automated, Fast PCR System for Point-Of-Care Molecular Diagnosis of Bacterial Infection

by
Dongkyu Lee
1,
Deawook Kim
1,2,
Jounghyuk Han
1,2,
Jongsu Yun
1,
Kang-Ho Lee
1,
Gyu Man Kim
2,
Ohwon Kwon
1,* and
Jaejong Lee
3,*
1
Daegu Research Center for Medical Devices, Korea Institute of Machinery and Materials, Daegu 42994, Korea
2
Department of Mechanical Engineering, Kyungpook National University, Daegu 41566, Korea
3
Nano-Mechanical Systems, Korea Institute of Machinery and Materials (KIMM), Daejeon 34103, Korea
*
Authors to whom correspondence should be addressed.
Sensors 2021, 21(2), 377; https://doi.org/10.3390/s21020377
Submission received: 12 November 2020 / Revised: 23 December 2020 / Accepted: 3 January 2021 / Published: 7 January 2021
(This article belongs to the Special Issue Advanced Biosensors for Bacterial Detection)
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Abstract

We developed an integrated PCR system that performs automated sample preparation and fast polymerase chain reaction (PCR) for application in point-of care (POC) testing. This system is assembled from inexpensive 3D-printing parts, off-the-shelf electronics and motors. Molecular detection requires a series of procedures including sample preparation, amplification, and fluorescence intensity analysis. The system can perform automated DNA sample preparation (extraction, separation and purification) in ≤5 min. The variance of the automated sample preparation was clearly lower than that achieved using manual DNA extraction. Fast thermal ramp cycles were generated by a customized thermocycler designed to automatically transport samples between heating and cooling blocks. Despite the large sample volume (50 μL), rapid two-step PCR amplification completed 40 cycles in ≤13.8 min. Variations in fluorescence intensity were measured by analyzing fluorescence images. As proof of concept of this system, we demonstrated the rapid DNA detection of pathogenic bacteria. We also compared the sensitivity of this system with that of a commercial device during the automated extraction and fast PCR of Salmonella bacteria.
Keywords: fast PCR; automated system; molecular diagnostics; sample preparation; rapid thermocycle fast PCR; automated system; molecular diagnostics; sample preparation; rapid thermocycle

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MDPI and ACS Style

Lee, D.; Kim, D.; Han, J.; Yun, J.; Lee, K.-H.; Kim, G.M.; Kwon, O.; Lee, J. Integrated, Automated, Fast PCR System for Point-Of-Care Molecular Diagnosis of Bacterial Infection. Sensors 2021, 21, 377. https://doi.org/10.3390/s21020377

AMA Style

Lee D, Kim D, Han J, Yun J, Lee K-H, Kim GM, Kwon O, Lee J. Integrated, Automated, Fast PCR System for Point-Of-Care Molecular Diagnosis of Bacterial Infection. Sensors. 2021; 21(2):377. https://doi.org/10.3390/s21020377

Chicago/Turabian Style

Lee, Dongkyu, Deawook Kim, Jounghyuk Han, Jongsu Yun, Kang-Ho Lee, Gyu Man Kim, Ohwon Kwon, and Jaejong Lee. 2021. "Integrated, Automated, Fast PCR System for Point-Of-Care Molecular Diagnosis of Bacterial Infection" Sensors 21, no. 2: 377. https://doi.org/10.3390/s21020377

APA Style

Lee, D., Kim, D., Han, J., Yun, J., Lee, K.-H., Kim, G. M., Kwon, O., & Lee, J. (2021). Integrated, Automated, Fast PCR System for Point-Of-Care Molecular Diagnosis of Bacterial Infection. Sensors, 21(2), 377. https://doi.org/10.3390/s21020377

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