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Article

Development of an Aptamer Based Luminescent Optical Fiber Sensor for the Continuous Monitoring of Hg2+ in Aqueous Media

1
Department of Electrical, Electronic and Communications Engineering, Public University of Navarra, Ed. Los Tejos, Campus Arrosadía s/n, E-31006 Pamplona, Navarra, Spain
2
Institute of Smart Cities, Public University of Navarra, Ed. Jerónimo de Ayanz, Campus Arrosadía s/n, E-31006 Pamplona, Navarra, Spain
*
Author to whom correspondence should be addressed.
Sensors 2020, 20(8), 2372; https://doi.org/10.3390/s20082372
Received: 22 March 2020 / Revised: 16 April 2020 / Accepted: 20 April 2020 / Published: 22 April 2020
(This article belongs to the Special Issue Calibration of Chemical Sensors Based on Photoluminescence)
A fluorescent optical fiber sensor for the detection of mercury (Hg2+) ions in aqueous solutions is presented in this work. The sensor was based on a fluorophore-labeled thymine (T)-rich oligodeoxyribonucleotide (ON) sequence that was directly immobilized onto the tip of a tapered optical fiber. In the presence of mercury ions, the formation of T–Hg2+-T mismatches quenches the fluorescence emission by the labeled fluorophore, which enables the measurement of Hg2+ ions in aqueous solutions. Thus, in contrast to commonly designed sensors, neither a fluorescence quencher nor a complementary ON sequence is required. The sensor presented a response time of 24.8 seconds toward 5 × 10−12 M Hg2+. It also showed both good reversibility (higher than the 95.8%) and selectivity: the I0/I variation was 10 times higher for Hg2+ ions than for Mn2+ ions. Other contaminants examined (Co2+, Ag+, Cd2+, Ni2+, Ca2+, Pb2+, Mn2+, Zn2+, Fe3+, and Cu2+) presented an even lower interference. The limit of detection of the sensor was 4.73 × 10−13 M Hg2+ in buffer solution and 9.03 × 10−13 M Hg2+ in ultrapure water, and was also able to detect 5 × 10−12 M Hg2+ in tap water. View Full-Text
Keywords: optical fiber biosensor; luminescent biosensor; fluorophore-labelled aptamer; mercury detection optical fiber biosensor; luminescent biosensor; fluorophore-labelled aptamer; mercury detection
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MDPI and ACS Style

De Acha, N.; Elosúa, C.; Arregui, F.J. Development of an Aptamer Based Luminescent Optical Fiber Sensor for the Continuous Monitoring of Hg2+ in Aqueous Media. Sensors 2020, 20, 2372. https://doi.org/10.3390/s20082372

AMA Style

De Acha N, Elosúa C, Arregui FJ. Development of an Aptamer Based Luminescent Optical Fiber Sensor for the Continuous Monitoring of Hg2+ in Aqueous Media. Sensors. 2020; 20(8):2372. https://doi.org/10.3390/s20082372

Chicago/Turabian Style

De Acha, Nerea, César Elosúa, and Francisco J. Arregui 2020. "Development of an Aptamer Based Luminescent Optical Fiber Sensor for the Continuous Monitoring of Hg2+ in Aqueous Media" Sensors 20, no. 8: 2372. https://doi.org/10.3390/s20082372

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