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Open AccessArticle

Fluorescence Sensing of Caffeine in Tea Beverages with 3,5-diaminobenzoic Acid

by Chenxu Du 1, Chaoqun Ma 1,2, Jiao Gu 1,2, Lei Li 1 and Guoqing Chen 1,2,*
1
School of Science, Jiangnan University, Wuxi 214122, China
2
Jiangsu Provincial Research Center of Light Industrial Optoelectronic Engineering and Technology, Wuxi 214122, China
*
Author to whom correspondence should be addressed.
Sensors 2020, 20(3), 819; https://doi.org/10.3390/s20030819
Received: 25 December 2019 / Revised: 21 January 2020 / Accepted: 31 January 2020 / Published: 3 February 2020
(This article belongs to the Special Issue Fluorescence-Based Sensors)
A rapid, selective and sensitive method for the detection of caffeine in tea infusion and tea beverages are proposed by using 3,5-diaminobenzoic acid as a fluorescent probe. The 3,5-diaminobenzoic acid emits strong fluorescence around 410 nm under the excitation of light at 280 nm. Both the molecular electrostatic potential analysis and fluorescent lifetime measurement proved that the existence of caffeine can quench the fluorescence of 3,5-diaminobenzoic acid. Under the optimal experimental parameters, the 3,5-diaminobenzoic acid was used as a fluorescent probe to detect the caffeine aqueous solution. There exists a good linear relationship between the fluorescence quenching of the fluorescent probe and the concentration of caffeine in the range of 0.1–100 μM, with recovery within 96.0 to 106.2%, while the limit of detection of caffeine is 0.03 μM. This method shows a high selectivity for caffeine. The caffeine content in different tea infusions and tea beverages has been determined and compared with the results from HPLC measurement. View Full-Text
Keywords: 3,5-diaminobenzoic acid; fluorescence probe; molecular electrostatic potential; caffeine 3,5-diaminobenzoic acid; fluorescence probe; molecular electrostatic potential; caffeine
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MDPI and ACS Style

Du, C.; Ma, C.; Gu, J.; Li, L.; Chen, G. Fluorescence Sensing of Caffeine in Tea Beverages with 3,5-diaminobenzoic Acid. Sensors 2020, 20, 819.

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