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Article

An Electrochemical Immunosensor Based on a Self-Assembled Monolayer Modified Electrode for Label-Free Detection of α-Synuclein

1
Department of Energy and Materials, Konkuk University, Chungju 380-701, Korea
2
Department of Chemistry and Chemical Engineering, Henan Institute of Science and Technology, Xinxiang 453003, China
3
Department of Liberal Arts, Konkuk University, Chungju-si 27478, Korea
*
Author to whom correspondence should be addressed.
Both authors contributed equally to this work.
Sensors 2020, 20(3), 617; https://doi.org/10.3390/s20030617
Received: 11 December 2019 / Revised: 18 January 2020 / Accepted: 19 January 2020 / Published: 22 January 2020
(This article belongs to the Section Chemical Sensors)
This research demonstrated the development of a simple, cost-effective, and label-free immunosensor for the detection of α-synuclein (α-Syn) based on a cystamine (CYS) self-assembled monolayer (SAM) decorated fluorine-doped tin oxide (FTO) electrode. CYS-SAM was formed onto the FTO electrode by the adsorption of CYS molecules through the head sulfur groups. The free amine (–NH2) groups at the tail of the CYS-SAM enabled the immobilization of anti-α-Syn-antibody, which concurrently allowed the formation of immunocomplex by covalent bonding with α-Syn-antigen. The variation of the concentrations of the attached α-Syn at the immunosensor probe induced the alternation of the current and the charge transfer resistance (Rct) for the redox response of [Fe(CN)6]3−/4−, which displayed a linear dynamic range from 10 to 1000 ng/mL with a low detection limit (S/N = 3) of ca. 3.62 and 1.13 ng/mL in differential pulse voltammetry (DPV) and electrochemical impedance spectra (EIS) measurements, respectively. The immunosensor displayed good reproducibility, anti-interference ability, and good recoveries of α-Syn detection in diluted human serum samples. The proposed immunosensor is a promising platform to detect α-Syn for the early diagnose of Parkinson’s disease, which can be extended for the determination of other biologically important biomarkers. View Full-Text
Keywords: α-synuclein; cystamine; immunosensor; self-assembled monolayer; fluorine-doped tin oxide α-synuclein; cystamine; immunosensor; self-assembled monolayer; fluorine-doped tin oxide
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MDPI and ACS Style

Ge, C.-Y.; Rahman, M.M.; Zhang, W.; Lopa, N.S.; Jin, L.; Yoon, S.; Jang, H.; Xu, G.-R.; Kim, W. An Electrochemical Immunosensor Based on a Self-Assembled Monolayer Modified Electrode for Label-Free Detection of α-Synuclein. Sensors 2020, 20, 617. https://doi.org/10.3390/s20030617

AMA Style

Ge C-Y, Rahman MM, Zhang W, Lopa NS, Jin L, Yoon S, Jang H, Xu G-R, Kim W. An Electrochemical Immunosensor Based on a Self-Assembled Monolayer Modified Electrode for Label-Free Detection of α-Synuclein. Sensors. 2020; 20(3):617. https://doi.org/10.3390/s20030617

Chicago/Turabian Style

Ge, Chuang-Ye, Md. M. Rahman, Wei Zhang, Nasrin S. Lopa, Lei Jin, Sujin Yoon, Hohyoun Jang, Guang-Ri Xu, and Whangi Kim. 2020. "An Electrochemical Immunosensor Based on a Self-Assembled Monolayer Modified Electrode for Label-Free Detection of α-Synuclein" Sensors 20, no. 3: 617. https://doi.org/10.3390/s20030617

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