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Communication

A Multichannel Pattern-Recognition-Based Protein Sensor with a Fluorophore-Conjugated Single-Stranded DNA Set

by 1,2,†, 1,†, 1,3,* and 1,3,*
1
Health and Medical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan
2
Faculty of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8573, Japan
3
DAILAB, DBT-AIST International Centre for Translational and Environmental Research (DAICENTER), National Institute of Advanced Industrial Science and Technology (AIST), Central 5-41, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Sensors 2020, 20(18), 5110; https://doi.org/10.3390/s20185110
Received: 30 July 2020 / Revised: 4 September 2020 / Accepted: 5 September 2020 / Published: 8 September 2020
(This article belongs to the Special Issue Nanosensors for Biomedical Applications)
Recently, pattern-recognition-based protein sensing has received considerable attention because it offers unique opportunities that complement more conventional antibody-based detection methods. Here, we report a multichannel pattern-recognition-based sensor using a set of fluorophore-conjugated single-stranded DNAs (ssDNAs), which can detect various proteins. Three different fluorophore-conjugated ssDNAs were placed into a single microplate well together with a target protein, and the generated optical response pattern that corresponds to each environment-sensitive fluorophore was read via multiple detection channels. Multivariate analysis of the resulting optical response patterns allowed an accurate detection of eight different proteases, indicating that fluorescence signal acquisition from a single compartment containing a mixture of ssDNAs is an effective strategy for the characterization of the target proteins. Additionally, the sensor could identify proteins, which are potential targets for disease diagnosis, in a protease and inhibitor mixture of different composition ratios. As our sensor benefits from simple construction and measurement procedures, and uses accessible materials, it offers a rapid and simple platform for the detection of proteins. View Full-Text
Keywords: biosensors; ssDNAs; multivariate analysis; pattern recognition; proteins biosensors; ssDNAs; multivariate analysis; pattern recognition; proteins
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MDPI and ACS Style

Okada, M.; Sugai, H.; Tomita, S.; Kurita, R. A Multichannel Pattern-Recognition-Based Protein Sensor with a Fluorophore-Conjugated Single-Stranded DNA Set. Sensors 2020, 20, 5110. https://doi.org/10.3390/s20185110

AMA Style

Okada M, Sugai H, Tomita S, Kurita R. A Multichannel Pattern-Recognition-Based Protein Sensor with a Fluorophore-Conjugated Single-Stranded DNA Set. Sensors. 2020; 20(18):5110. https://doi.org/10.3390/s20185110

Chicago/Turabian Style

Okada, Mari, Hiroka Sugai, Shunsuke Tomita, and Ryoji Kurita. 2020. "A Multichannel Pattern-Recognition-Based Protein Sensor with a Fluorophore-Conjugated Single-Stranded DNA Set" Sensors 20, no. 18: 5110. https://doi.org/10.3390/s20185110

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