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Protein Determination with Molecularly Imprinted Polymer Recognition Combined with Birefringence Liquid Crystal Detection

Institute of Physical Chemistry Polish Academy of Sciences, Kasprzaka Str. 44/52, 01-224 Warsaw, Poland
Faculty of Advanced Technologies and Chemistry, Military University of Technology, Kaliskiego Str. 2, 00-908 Warsaw, Poland
Department of Chemistry, University of North Texas, 1155 Union Circle No 305070, Denton, TX 76203-5017, USA
Faculty of Mathematics and Natural Sciences, School of Sciences, Cardinal Stefan Wyszynski University in Warsaw, Wóycickiego Str. 1/3, 01-815 Warsaw, Poland
Author to whom correspondence should be addressed.
Both authors contributed equally to this work.
Sensors 2020, 20(17), 4692;
Received: 18 July 2020 / Revised: 14 August 2020 / Accepted: 17 August 2020 / Published: 20 August 2020
(This article belongs to the Special Issue Sensors for Environmental and Life Science Applications)
Liquid crystal-based sensors offer the advantage of high sensitivity at a low cost. However, they often lack selectivity altogether or require costly and unstable biomaterials to impart this selectivity. To incur this selectivity, we herein integrated a molecularly imprinted polymer (MIP) film recognition unit with a liquid crystal (LC) in an optical cell transducer. We tested the resulting chemosensor for protein determination. We examined two different LCs, each with a different optical birefringence. That way, we revealed the influence of that parameter on the sensitivity of the (human serum albumin)-templated (MIP-HSA) LC chemosensor. The response of this chemosensor with the (MIP-HSA)-recognizing film was linear from 2.2 to 15.2 µM HSA, with a limit of detection of 2.2 µM. These values are sufficient to use the devised chemosensor for HSA determination in biological samples. Importantly, the imprinting factor (IF) of this chemosensor was appreciable, reaching IF = 3.7. This IF value indicated the predominant binding of the HSA through specific rather than nonspecific interactions with the MIP. View Full-Text
Keywords: molecularly imprinted polymer; liquid crystal; human serum albumin molecularly imprinted polymer; liquid crystal; human serum albumin
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Cieplak, M.; Węgłowski, R.; Iskierko, Z.; Węgłowska, D.; Sharma, P.S.; Noworyta, K.R.; D’Souza, F.; Kutner, W. Protein Determination with Molecularly Imprinted Polymer Recognition Combined with Birefringence Liquid Crystal Detection. Sensors 2020, 20, 4692.

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