In Vivo Tumor Vascular Imaging with Light Emitting Diode-Based Photoacoustic Imaging System
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Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA
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Research & Business Development Division, Cyberdyne INC, Cambridge Innovation Center, 3013 Rotterdam, The Netherlands
3
Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
*
Author to whom correspondence should be addressed.
Sensors 2020, 20(16), 4503; https://doi.org/10.3390/s20164503
Received: 23 July 2020
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Revised: 7 August 2020
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Accepted: 8 August 2020
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Published: 12 August 2020
(This article belongs to the Special Issue Biomedical Photoacoustic Imaging and Sensing Using Affordable Resources)
Photoacoustic (PA) imaging has shown tremendous promise for imaging tumor vasculature and its function at deeper penetration depths without the use of exogenous contrast agents. Traditional PA imaging systems employ expensive and bulky class IV lasers with low pulse repetition rate, due to which its availability for preclinical cancer research is hampered. In this study, we evaluated the capability of a Light-Emitting Diode (LED)-based PA and ultrasound (US) imaging system for monitoring heterogeneous microvasculature in tumors (up to 10 mm in depth) and quantitatively compared the PA images with gold standard histology images. We used a combination of a 7 MHz linear array US transducer and 850 nm excitation wavelength LED arrays to image blood vessels in a subcutaneous tumor model. After imaging, the tumors were sectioned and stained for endothelial cells to correlate with PA images across similar cross-sections. Analysis of 30 regions of interest in tumors from different mice showed a statistically significant R-value of 0.84 where the areas with high blood vessel density had high PA response while low blood vessel density regions had low PA response. Our results confirm that LED-based PA and US imaging can provide 2D and 3D images of tumor vasculature and the potential it has as a valuable tool for preclinical cancer research.
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Keywords:
LED; photoacoustic imaging; ultrasound; tumor imaging
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MDPI and ACS Style
Xavierselvan, M.; Singh, M.K.A.; Mallidi, S. In Vivo Tumor Vascular Imaging with Light Emitting Diode-Based Photoacoustic Imaging System. Sensors 2020, 20, 4503. https://doi.org/10.3390/s20164503
AMA Style
Xavierselvan M, Singh MKA, Mallidi S. In Vivo Tumor Vascular Imaging with Light Emitting Diode-Based Photoacoustic Imaging System. Sensors. 2020; 20(16):4503. https://doi.org/10.3390/s20164503
Chicago/Turabian StyleXavierselvan, Marvin, Mithun K.A. Singh, and Srivalleesha Mallidi. 2020. "In Vivo Tumor Vascular Imaging with Light Emitting Diode-Based Photoacoustic Imaging System" Sensors 20, no. 16: 4503. https://doi.org/10.3390/s20164503
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