Next Article in Journal
Stone Paper as a New Substrate to Fabricate Flexible Screen-Printed Electrodes for the Electrochemical Detection of Dopamine
Previous Article in Journal
QCL-Based Dual-Comb Spectrometer for Multi-Species Measurements at High Temperatures and High Pressures
Open AccessArticle

Advantages of Highly Spherical Gold Nanoparticles as Labels for Lateral Flow Immunoassay

1
A.N. Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences, Moscow 119071, Russia
2
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov 410049, Russia
3
Saratov State University, Saratov 410012, Russia
*
Authors to whom correspondence should be addressed.
Sensors 2020, 20(12), 3608; https://doi.org/10.3390/s20123608
Received: 15 May 2020 / Revised: 20 June 2020 / Accepted: 23 June 2020 / Published: 26 June 2020
(This article belongs to the Special Issue Biomedical Optical Nanosensors)
The use of lateral flow immunoassays (LFIAs) for rapid on-site testing is restricted by their relatively high limit of detection (LoD). One possible way to decrease the LoD is to optimize nanoparticle properties that are used as labels. We compare two types of Au nanoparticles: usual quasispherical gold nanoparticles (C-GNPs), obtained by the Turkevich–Frens method, and superspherical gold nanoparticles (S-GNPs), obtained by a progressive overgrowth technique. Average diameters were 18.6–47.5 nm for C-GNPs and 20.2–90.4 nm for S-GNPs. Cardiomarker troponin I was considered as the target analyte. Adsorption and covalent conjugation with antibodies were tested for both GNP types. For C-GNPs, the minimal LoD was obtained with 33.7 nm nanoparticles, reaching 12.7 ng/mL for covalent immobilization and 9.9 ng/mL for adsorption. The average diameter of S-GNPs varied from 20.2 to 64.5 nm, which resulted in a decrease in LoD for an LFIA of troponin I from 3.4 to 1.2 ng/mL for covalent immobilization and from 2.9 to 2.0 ng/mL for adsorption. Thus, we obtained an 8-fold decrease in LoD (9.9 to 1.2 ng/mL) by using S-GNPs. This effect can be related to more effective antibody immobilization and improved S-GNP optical properties. The obtained results can improve LFIAs for various practically significant analytes. View Full-Text
Keywords: immunochromatography; nanosized labels; nanoparticle–antibody conjugates; assay sensitivity; cardiomarker immunochromatography; nanosized labels; nanoparticle–antibody conjugates; assay sensitivity; cardiomarker
Show Figures

Figure 1

MDPI and ACS Style

Byzova, N.A.; Zherdev, A.V.; Khlebtsov, B.N.; Burov, A.M.; Khlebtsov, N.G.; Dzantiev, B.B. Advantages of Highly Spherical Gold Nanoparticles as Labels for Lateral Flow Immunoassay. Sensors 2020, 20, 3608.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop