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Open AccessArticle

Application of a Waveguide-Mode Sensor to Blood Testing for Hepatitis B Virus, Hepatitis C Virus, Human Immunodeficiency Virus and Treponema pallidum Infection

1
Division of Biochemistry, Department of Biomedical Sciences, Nihon University School of Medicine, Itabashi-ku, Tokyo 173-8610, Japan
2
Electronics and Photonics Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan
3
Department of Life Science & Green Chemistry, Saitama Institute of Technology, 1690 Fusaiji, Fukaya, Saitama 369-0293, Japan
*
Author to whom correspondence should be addressed.
Present address: Sensing System Research Center, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
Sensors 2019, 19(7), 1729; https://doi.org/10.3390/s19071729
Received: 28 February 2019 / Revised: 28 March 2019 / Accepted: 8 April 2019 / Published: 11 April 2019
(This article belongs to the Special Issue Optical Bio Sensing)
Testing for blood-transmitted infectious agents is an important aspect of safe medical treatment. During emergencies, such as significant earthquakes, many patients need surgical treatment and/or blood transfusion. Because a waveguide mode (WM) sensor can be used as a portable, on-site blood testing device in emergency settings, we have previously developed WM sensors for detection of antibodies against hepatitis B virus and hepatitis C virus and for forward ABO and Rh(D) and reverse ABO blood typing. In this study, we compared signal enhancement methods using secondary antibodies conjugated with peroxidase, a fluorescent dye, and gold nanoparticles, and found that the peroxidase reaction method offers superior sensitivity while gold nanoparticles provide the most rapid detection of anti-HBs antibody. Next, we examined whether we could apply a WM sensor with signal enhancement with peroxidase or gold nanoparticles to detection of antibodies against hepatitis C virus, human immunodeficiency virus and Treponema pallidum, and HBs antigen in plasma. We showed that a WM sensor can detect significant signals of these infectious agents within 30 min. Therefore, a portable device utilizing a WM sensor can be used for on-site blood testing of infectious agents in emergency settings. View Full-Text
Keywords: waveguide-mode sensor; on-site blood testing; hepatitis B virus; hepatitis C virus; human immunodeficiency virus; Treponema pallidum waveguide-mode sensor; on-site blood testing; hepatitis B virus; hepatitis C virus; human immunodeficiency virus; Treponema pallidum
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MDPI and ACS Style

Uno, S.; Shimizu, T.; Tanaka, T.; Ashiba, H.; Fujimaki, M.; Tanaka, M.; Awazu, K.; Makishima, M. Application of a Waveguide-Mode Sensor to Blood Testing for Hepatitis B Virus, Hepatitis C Virus, Human Immunodeficiency Virus and Treponema pallidum Infection. Sensors 2019, 19, 1729. https://doi.org/10.3390/s19071729

AMA Style

Uno S, Shimizu T, Tanaka T, Ashiba H, Fujimaki M, Tanaka M, Awazu K, Makishima M. Application of a Waveguide-Mode Sensor to Blood Testing for Hepatitis B Virus, Hepatitis C Virus, Human Immunodeficiency Virus and Treponema pallidum Infection. Sensors. 2019; 19(7):1729. https://doi.org/10.3390/s19071729

Chicago/Turabian Style

Uno, Shigeyuki; Shimizu, Takenori; Tanaka, Torahiko; Ashiba, Hiroki; Fujimaki, Makoto; Tanaka, Mutsuo; Awazu, Koichi; Makishima, Makoto. 2019. "Application of a Waveguide-Mode Sensor to Blood Testing for Hepatitis B Virus, Hepatitis C Virus, Human Immunodeficiency Virus and Treponema pallidum Infection" Sensors 19, no. 7: 1729. https://doi.org/10.3390/s19071729

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