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Development of a Novel Enhanced Biosensor System for Real-Time Monitoring of Fish Stress Using a Self-Assembled Monolayer

1
Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Tokyo 1088477, Japan
2
Graduate School of Agricultural Science, Tohoku University, Sendai-shi 9808572, Japan
3
Hokkaido Industrial Technology Center, Hakodate-shi 0410801, Japan
4
National Research Institute of Fisheries and Environment of Inland Sea, Japan Fisheries Research and Education Agency, Takamatsu-shi 761-0111, Japan
*
Author to whom correspondence should be addressed.
Sensors 2019, 19(7), 1518; https://doi.org/10.3390/s19071518
Received: 17 January 2019 / Revised: 12 March 2019 / Accepted: 26 March 2019 / Published: 28 March 2019
(This article belongs to the Special Issue Enzyme-Based Biosensors for Biomedical Analysis)
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Abstract

Wireless biosensor systems were developed in our lab for monitoring blood glucose concentrations in fish as an indicator of fish stress. However, uniform immobilization of the enzyme on the surface of the electrode is difficult, so the sensor response is typically reduced at a range of high glucose concentrations during the stress monitoring. In this study, we attempted to enhance sensor response by using a self-assembled monolayer-immobilized enzyme. Glucose oxidase was immobilized on a working electrode modified with a self-assembled monolayer. The proposed biosensor showed a good correlation between the output current and the glucose concentration range of 10–3500 mg dL−1 under an optimized working condition. The dynamic measurement range of this newly developed sensor is significantly improved, especially over a high concentration range, which helps the sensor to achieve better performance in dramatic changes in the stress response of fish. In addition, we used biological samples from test fish and obtained a good correlation coefficient between the sensor output current and the glucose concentration using a conventional method. The proposed wireless biosensor system was also applied to monitor fish stress responses in real time through different stressors and to obtain some precise data that reflect real fish stress responses. View Full-Text
Keywords: biosensor; real-time monitoring; fish; stress; self-assembled monolayer; glucose biosensor; real-time monitoring; fish; stress; self-assembled monolayer; glucose
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Wu, H.; Fujii, Y.; Nakano, T.; Arimoto, T.; Murata, M.; Matsumoto, H.; Yoshiura, Y.; Ohnuki, H.; Endo, H. Development of a Novel Enhanced Biosensor System for Real-Time Monitoring of Fish Stress Using a Self-Assembled Monolayer. Sensors 2019, 19, 1518.

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