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Open AccessFeature PaperArticle

Biocompatible Cantilevers for Mechanical Characterization of Zebrafish Embryos using Image Analysis

1
Department of Biomedical Engineering, University of Connecticut, Storrs, CT 06269, USA
2
Department of Molecular & Cell Biology, University of Connecticut, Storrs, CT 06269, USA
*
Author to whom correspondence should be addressed.
Sensors 2019, 19(7), 1506; https://doi.org/10.3390/s19071506
Received: 15 February 2019 / Revised: 15 March 2019 / Accepted: 22 March 2019 / Published: 28 March 2019
(This article belongs to the Special Issue Cantilever Sensor)
We have developed a force sensing system to continuously evaluate the mechanical elasticity of micrometer-scale (a few hundred micrometers to a millimeter) live tissues. The sensing is achieved by measuring the deflection of force sensitive cantilevers through microscopic image analysis, which does not require electrical strain gauges. Cantilevers made of biocompatible polydimethylsiloxane (PDMS) were actuated by a piezoelectric actuator and functioned as a pair of chopsticks to measure the stiffness of the specimen. The dimensions of the cantilevers were easily adjusted to match the size, range, and stiffness of the zebrafish samples. In this paper, we demonstrated the versatility of this technique by measuring the mechanical elasticity of zebrafish embryos at different stages of development. The stiffness of zebrafish embryos was measured once per hour for 9 h. From the experimental results, we successfully quantified the stiffness change of zebrafish embryos during embryonic development. View Full-Text
Keywords: stiffness analysis; force sensor; zebrafish embryo; biosolid mechanics; soft lithography stiffness analysis; force sensor; zebrafish embryo; biosolid mechanics; soft lithography
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MDPI and ACS Style

Tomizawa, Y.; Dixit, K.; Daggett, D.; Hoshino, K. Biocompatible Cantilevers for Mechanical Characterization of Zebrafish Embryos using Image Analysis. Sensors 2019, 19, 1506.

AMA Style

Tomizawa Y, Dixit K, Daggett D, Hoshino K. Biocompatible Cantilevers for Mechanical Characterization of Zebrafish Embryos using Image Analysis. Sensors. 2019; 19(7):1506.

Chicago/Turabian Style

Tomizawa, Yuji; Dixit, Krishna; Daggett, David; Hoshino, Kazunori. 2019. "Biocompatible Cantilevers for Mechanical Characterization of Zebrafish Embryos using Image Analysis" Sensors 19, no. 7: 1506.

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