Next Article in Journal
A Fetal ECG Monitoring System Based on the Android Smartphone
Previous Article in Journal
Contactless In Situ Electrical Characterization Method of Printed Electronic Devices with Terahertz Spectroscopy
Previous Article in Special Issue
An Ultra-Rapid Biosensory Point-of-Care (POC) Assay for Prostate-Specific Antigen (PSA) Detection in Human Serum
Open AccessArticle

A Point-of-Care Device for Molecular Diagnosis Based on CMOS SPAD Detectors with Integrated Microfluidics

Electronic and Biomedical Engineering Department, University of Barcelona, 08028 Barcelona, Spain
*
Author to whom correspondence should be addressed.
Sensors 2019, 19(3), 445; https://doi.org/10.3390/s19030445
Received: 3 December 2018 / Revised: 17 January 2019 / Accepted: 17 January 2019 / Published: 22 January 2019
(This article belongs to the Special Issue Point of Care Sensors)
We describe the integration of techniques and technologies to develop a Point-of-Care for molecular diagnosis PoC-MD, based on a fluorescence lifetime measurement. Our PoC-MD is a low-cost, simple, fast, and easy-to-use general-purpose platform, aimed at carrying out fast diagnostics test through label detection of a variety of biomarkers. It is based on a 1-D array of 10 ultra-sensitive Single-Photon Avalanche Diode (SPAD) detectors made in a 0.18 μm High-Voltage Complementary Metal Oxide Semiconductor (HV-CMOS) technology. A custom microfluidic polydimethylsiloxane cartridge to insert the sample is straightforwardly positioned on top of the SPAD array without any alignment procedure with the SPAD array. Moreover, the proximity between the sample and the gate-operated SPAD sensor makes unnecessary any lens or optical filters to detect the fluorescence for long lifetime fluorescent dyes, such as quantum dots. Additionally, the use of a low-cost laser diode as pulsed excitation source and a Field-Programmable Gate Array (FPGA) to implement the control and processing electronics, makes the device flexible and easy to adapt to the target label molecule by only changing the laser diode. Using this device, reliable and sensitive real-time proof-of-concept fluorescence lifetime measurement of quantum dot QdotTM 605 streptavidin conjugate is demonstrated. View Full-Text
Keywords: fluorescence lifetime; low-cost; molecular diagnosis; microfluidics; point-of-care; CMOS; single-photon avalanche diode; laser diode fluorescence lifetime; low-cost; molecular diagnosis; microfluidics; point-of-care; CMOS; single-photon avalanche diode; laser diode
Show Figures

Figure 1

MDPI and ACS Style

Canals, J.; Franch, N.; Alonso, O.; Vilà, A.; Diéguez, A. A Point-of-Care Device for Molecular Diagnosis Based on CMOS SPAD Detectors with Integrated Microfluidics. Sensors 2019, 19, 445.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop