A Point-of-Care Device for Molecular Diagnosis Based on CMOS SPAD Detectors with Integrated Microfluidics
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Measuring Technique
2.3. Device Implementation
2.3.1. SPAD Sensor Chip Design
2.3.2. Sensor Packaging
2.3.3. Microfluidic Cartridge Fabrication
2.3.4. Excitation Source
2.3.5. System Controller
2.3.6. System Configuration
3. Results
3.1. Sensor Characterization
3.2. System Performance
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Components | Approx. Cost | Cost Per Device | Notes |
---|---|---|---|
SPAD sensor chip (40 dies fabricated in a multi-project wafer) | 7000 € | 175 € | |
Zedboard Zynq-7000 Development Board from Digilent | 422 € | 422 € | |
3D printed spacer (based on the 3D printer cartridge cost (15 €)) | 0.15 € | 0.15 € | |
Daughter card (includes all electronic parts and components) | 110 € | 110 € | |
Sensor Packaging | |||
Substrate based on a printed circuit board | 66 € | 66 € | |
Conductive epoxy CW2400 from Chemtronics Circuit Works | 100 € | 2 € | |
SU-8 100 negative tone near UV photoresist from Microchem Corporation | 860 € | 8.6 € | 1 |
Laser driver circuit components | |||
Laser driver circuit DC supply for V+ (includes all electronics parts and components) | 96 € | 96 € | |
Laser diode 405 nm and 150 mW L405P150 from Thorlabs | 83 € | 83 € | |
Microfluidics materials | |||
Polydimethylsiloxane—PDMS, Dow Corning Sylgard 184 kits | 170 € | 3.4 € | 2 |
Glass coverslip 12460S from Thermo Fisher Scientific (1000 units) | 55 € | 0.055 € | |
Fluorophore label | |||
Q10103MP 50 μL 1 μM QdotTM 605 Streptavidin Conjugate from Thermo Fisher Scientific | 203 € | ||
PBS PH7.4 W/O CAMG USA PLASTIC 500 mL | 12 € |
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Canals, J.; Franch, N.; Alonso, O.; Vilà, A.; Diéguez, A. A Point-of-Care Device for Molecular Diagnosis Based on CMOS SPAD Detectors with Integrated Microfluidics. Sensors 2019, 19, 445. https://doi.org/10.3390/s19030445
Canals J, Franch N, Alonso O, Vilà A, Diéguez A. A Point-of-Care Device for Molecular Diagnosis Based on CMOS SPAD Detectors with Integrated Microfluidics. Sensors. 2019; 19(3):445. https://doi.org/10.3390/s19030445
Chicago/Turabian StyleCanals, Joan, Nil Franch, Oscar Alonso, Anna Vilà, and Angel Diéguez. 2019. "A Point-of-Care Device for Molecular Diagnosis Based on CMOS SPAD Detectors with Integrated Microfluidics" Sensors 19, no. 3: 445. https://doi.org/10.3390/s19030445