Development of Human Serum Albumin Selective Fluorescent Probe Using Thieno[3,2-b]pyridine-5(4H)-one Fluorophore Derivatives
by
Suji Lee 1, Dan-Bi Sung 2, Seungyoon Kang 1, Saravanan Parameswaran 1
, Jun-Ho Choi 1, Jong Seok Lee 2,3,* and Min Su Han 1,*
Suji Lee 1, Dan-Bi Sung 2, Seungyoon Kang 1, Saravanan Parameswaran 1, Jun-Ho Choi 1, Jong Seok Lee 2,3,* and Min Su Han 1,*
1
Department of Chemistry, Gwangju Institute of Science and Technology (GIST), 123 Cheomdangwagi-ro, Buk-gu, Gwangju 61005, Korea
2
Marine Natural Products Chemistry Laboratory, Korea Institute of Ocean Science and Technology (KIOST), Busan 49111, Korea
3
Department of Marine Biotechnology, Korea University of Science and Technology, Daejeon 34113, Korea
*
Authors to whom correspondence should be addressed.
Sensors 2019, 19(23), 5298; https://doi.org/10.3390/s19235298
Received: 30 October 2019 / Revised: 19 November 2019 / Accepted: 28 November 2019 / Published: 1 December 2019
(This article belongs to the Section Chemical Sensors)
The level of human serum albumin (HSA) in biological fluids is a key health indicator and its quantitative determination has great clinical importance. In this study, we developed a selective and sensitive fluorescent HSA probe by fluorescence-based high-throughput screening of a set of fluorescent thieno[3,2-b]pyridine-5(4H)-one derivatives against major plasma proteins: HSA, bovine serum albumin (BSA), globulin, fibrinogen, and transferrin. The fluorophore chosen finally (4) showed noticeable fluorescence enhancement in the presence of HSA (160-fold increase), and it exhibited rapid response, high sensitivity (detection limit 8 nM), and the ability to clearly distinguish HSA from BSA in pH 9 buffer condition. Moreover, the probe could be applicable to detect trace amounts of HSA in an artificial urine sample; further, it might be applied to the determination of the HSA concentration in complex biological samples for pre-clinical diagnosis.
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Keywords:
human serum albumin; fluorescent probe; thieno[3,2-b]pyridine-5(4H)-one; microalbuminuria; intramolecular charge transfer
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
MDPI and ACS Style
Lee, S.; Sung, D.-B.; Kang, S.; Parameswaran, S.; Choi, J.-H.; Lee, J.S.; Han, M.S. Development of Human Serum Albumin Selective Fluorescent Probe Using Thieno[3,2-b]pyridine-5(4H)-one Fluorophore Derivatives. Sensors 2019, 19, 5298.
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