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Sensors
Volume 19
Issue 23
10.3390/s19235298
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Article

Development of Human Serum Albumin Selective Fluorescent Probe Using Thieno[3,2-b]pyridine-5(4H)-one Fluorophore Derivatives

by
Suji Lee
1,
Dan-Bi Sung
2,
Seungyoon Kang
1,
Saravanan Parameswaran
1,
Jun-Ho Choi
1,
Jong Seok Lee
2,3,* and
Min Su Han
1,*
1
Department of Chemistry, Gwangju Institute of Science and Technology (GIST), 123 Cheomdangwagi-ro, Buk-gu, Gwangju 61005, Korea
2
Marine Natural Products Chemistry Laboratory, Korea Institute of Ocean Science and Technology (KIOST), Busan 49111, Korea
3
Department of Marine Biotechnology, Korea University of Science and Technology, Daejeon 34113, Korea
*
Authors to whom correspondence should be addressed.
Sensors 2019, 19(23), 5298; https://doi.org/10.3390/s19235298
Received: 30 October 2019 / Revised: 19 November 2019 / Accepted: 28 November 2019 / Published: 1 December 2019
(This article belongs to the Section Chemical Sensors)
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Abstract

The level of human serum albumin (HSA) in biological fluids is a key health indicator and its quantitative determination has great clinical importance. In this study, we developed a selective and sensitive fluorescent HSA probe by fluorescence-based high-throughput screening of a set of fluorescent thieno[3,2-b]pyridine-5(4H)-one derivatives against major plasma proteins: HSA, bovine serum albumin (BSA), globulin, fibrinogen, and transferrin. The fluorophore chosen finally (4) showed noticeable fluorescence enhancement in the presence of HSA (160-fold increase), and it exhibited rapid response, high sensitivity (detection limit 8 nM), and the ability to clearly distinguish HSA from BSA in pH 9 buffer condition. Moreover, the probe could be applicable to detect trace amounts of HSA in an artificial urine sample; further, it might be applied to the determination of the HSA concentration in complex biological samples for pre-clinical diagnosis. View Full-Text
Keywords: human serum albumin; fluorescent probe; thieno[3,2-b]pyridine-5(4H)-one; microalbuminuria; intramolecular charge transfer human serum albumin; fluorescent probe; thieno[3,2-b]pyridine-5(4H)-one; microalbuminuria; intramolecular charge transfer
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Lee, S.; Sung, D.-B.; Kang, S.; Parameswaran, S.; Choi, J.-H.; Lee, J.S.; Han, M.S. Development of Human Serum Albumin Selective Fluorescent Probe Using Thieno[3,2-b]pyridine-5(4H)-one Fluorophore Derivatives. Sensors 2019, 19, 5298. https://doi.org/10.3390/s19235298

AMA Style

Lee S, Sung D-B, Kang S, Parameswaran S, Choi J-H, Lee JS, Han MS. Development of Human Serum Albumin Selective Fluorescent Probe Using Thieno[3,2-b]pyridine-5(4H)-one Fluorophore Derivatives. Sensors. 2019; 19(23):5298. https://doi.org/10.3390/s19235298

Chicago/Turabian Style

Lee, Suji, Dan-Bi Sung, Seungyoon Kang, Saravanan Parameswaran, Jun-Ho Choi, Jong Seok Lee, and Min Su Han. 2019. "Development of Human Serum Albumin Selective Fluorescent Probe Using Thieno[3,2-b]pyridine-5(4H)-one Fluorophore Derivatives" Sensors 19, no. 23: 5298. https://doi.org/10.3390/s19235298

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