Biosensors for the Detection of Interaction between Legionella pneumophila Collagen-Like Protein and Glycosaminoglycans
1
Department of Physical and Environmental Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON M1C 1A4, Canada
2
Department of Biological Sciences and Cell and Systems Biology, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON M1C 1A4, Canada
3
BIOASTER Microbiology Technology Institute, 40 Avenue Tony Garnier, 69007 Lyon, France
*
Author to whom correspondence should be addressed.
Sensors 2018, 18(8), 2668; https://doi.org/10.3390/s18082668
Received: 10 July 2018 / Revised: 8 August 2018 / Accepted: 10 August 2018 / Published: 14 August 2018
(This article belongs to the Special Issue State-of-the-Art Sensors Technology in Canada 2018)
The adhesin Legionella collagen-like (Lcl) protein can bind to extracellular matrix components and mediate the binding of Legionella pneumophila to host cells. In this study, electrochemical impedance spectroscopy (EIS) and surface plasmon resonance (SPR)-based biosensors were employed to characterize these interactions between glycosaminoglycans (GAGs) and the adhesin Lcl protein. Fucoidan displayed a high affinity (KD 18 nM) for Lcl protein. Chondroitin sulfate A and dermatan sulfate differ in the position of a carboxyl group replacing D-glucuronate with D-iduronate. Our results indicated that the presence of D-iduronate in dermatan sulfate strongly hindered its interaction with Lcl. These biophysical studies provided valuable information in our understanding of adhesin-ligand interactions related to Legionella pneumophila infections.
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Keywords:
Legionella collagen-like protein; glycosaminoglycans; fucoidan; surface plasmon resonance; electrochemical impedance spectroscopy
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
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MDPI and ACS Style
Su, H.; Li, S.; Terebiznik, M.; Guyard, C.; Kerman, K. Biosensors for the Detection of Interaction between Legionella pneumophila Collagen-Like Protein and Glycosaminoglycans. Sensors 2018, 18, 2668.
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