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Open AccessArticle

Prospective Use of High-Refractive Index Materials for Single Molecule Detection in Flow Cytometry

1
Vaccine Branch, Center for Cancer Research, National Institutes of Health, Bethesda, MD 20892, USA
2
Optoelectronics Research Centre, University of Southampton, Southampton SO17 1BJ, UK
*
Author to whom correspondence should be addressed.
Sensors 2018, 18(8), 2461; https://doi.org/10.3390/s18082461
Received: 23 April 2018 / Revised: 17 July 2018 / Accepted: 18 July 2018 / Published: 1 August 2018
(This article belongs to the Special Issue Biosensing for Interfacial Science)
Phenotyping extracellular vesicles (EVs), where surface receptor expression is often as low as one molecule per EV, remains problematic due to the inability of commercial flow cytometers to provide single-fluorescent molecule sensitivity. While EVs are widely considered to be of great potential as diagnostic, prognostic and theranostic biomarkers, their use is currently hindered by the lack of tools available to accurately and reproducibly enumerate and phenotype them. Herein, we propose a new class of labels that leverage the biophysical properties of materials with unique complex refractive indices and demonstrate that this class of labels has the possibility of allowing single-epitope detection using conventional flow cytometry. View Full-Text
Keywords: extracellular vesicles; flow cytometry; light scatter; scatter modelling; complex refractive index; scatter labels; NanoTags; single-epitope detection extracellular vesicles; flow cytometry; light scatter; scatter modelling; complex refractive index; scatter labels; NanoTags; single-epitope detection
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MDPI and ACS Style

Welsh, J.A.; Kepley, J.; Rosner, A.; Horak, P.; Berzofsky, J.A.; Jones, J.C. Prospective Use of High-Refractive Index Materials for Single Molecule Detection in Flow Cytometry. Sensors 2018, 18, 2461. https://doi.org/10.3390/s18082461

AMA Style

Welsh JA, Kepley J, Rosner A, Horak P, Berzofsky JA, Jones JC. Prospective Use of High-Refractive Index Materials for Single Molecule Detection in Flow Cytometry. Sensors. 2018; 18(8):2461. https://doi.org/10.3390/s18082461

Chicago/Turabian Style

Welsh, Joshua A.; Kepley, Julia; Rosner, Ariel; Horak, Peter; Berzofsky, Jay A.; Jones, Jennifer C. 2018. "Prospective Use of High-Refractive Index Materials for Single Molecule Detection in Flow Cytometry" Sensors 18, no. 8: 2461. https://doi.org/10.3390/s18082461

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