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Sensors 2018, 18(8), 2457; https://doi.org/10.3390/s18082457

Studies towards hcTnI Immunodetection Using Electrochemical Approaches Based on Magnetic Microbeads

1
Nanobiotechnology for Diagnostics (Nb4D), Department of Chemical and Biomolecular Nanotechnology, Institute for Advanced Chemistry of Catalonia (IQAC) of the Spanish Council for Scientific Research (CSIC), Jordi Girona 18-26, 08034 Barcelona, Spain
2
CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Jordi Girona 18-26, 08034 Barcelona, Spain
*
Author to whom correspondence should be addressed.
Received: 19 July 2018 / Revised: 26 July 2018 / Accepted: 27 July 2018 / Published: 29 July 2018
(This article belongs to the Special Issue Magnetic Materials Based Biosensors)
Full-Text   |   PDF [2289 KB, uploaded 29 July 2018]   |  

Abstract

Different electrochemical strategies based on the use of magnetic beads are described in this work for the detection of human cardiac troponin I (hcTnI). hcTnI is also known as the gold standard for acute myocardial infarction (AMI) diagnosis according to the different guidelines from the European Society of Cardiology (ESC) and the American College of Cardiology (ACC). Amperometric and voltamperometric sandwich magnetoimmunoassays were developed by biofunctionalization of paramagnetic beads with specific antibodies. These bioconjugates were combined with biotinylated antibodies as detection antibodies, with the aim of testing different electrochemical transduction principles. Streptavidin labeled with horseradish peroxidase was used for the amperometric magnetoimmunoassay, reaching a detectability of 0.005 ± 0.002 µg mL−1 in 30 min. Cadmium quantum dots-streptavidin bioconjugates were used in the case of the voltamperometric immunosensor reaching a detectability of 0.023 ± 0.014 µg mL−1. View Full-Text
Keywords: human cardiac troponin I; magnetic beads; magnetoimmunosensor; cadmium quantum dots; Streptavidin-Horseradish Peroxidase human cardiac troponin I; magnetic beads; magnetoimmunosensor; cadmium quantum dots; Streptavidin-Horseradish Peroxidase
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Hernández-Albors, A.; Colom, G.; Salvador, J.-P.; Marco, M.-P. Studies towards hcTnI Immunodetection Using Electrochemical Approaches Based on Magnetic Microbeads. Sensors 2018, 18, 2457.

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