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Sensors 2018, 18(12), 4324; https://doi.org/10.3390/s18124324

Development of Highly Sensitive Immunosensor for Clenbuterol Detection by Using Poly(3,4-ethylenedioxythiophene)/Graphene Oxide Modified Screen-Printed Carbon Electrode

1
Functional Devices Laboratory, Institute of Advanced Technology, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia
2
Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia
3
Biodiagnostic-Biosensor Programme, Biotechnology and Nanotechnology Research Centre, Malaysian Agricultural Research and Development Institute, Serdang 43400, Selangor, Malaysia
*
Author to whom correspondence should be addressed.
Received: 20 September 2018 / Revised: 29 October 2018 / Accepted: 3 November 2018 / Published: 7 December 2018
(This article belongs to the Section Biosensors)
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Abstract

Clenbuterol (CLB) is an antibiotic and illegal growth promoter drug that has a long half-life and easily remains as residue and contaminates the animal-based food product that leads to various health problems. In this work, electrochemical immunosensor based on poly(3,4-ethylenedioxythiophene)/graphene oxide (PEDOT/GO) modified screen-printed carbon electrode (SPCE) for CLB detection was developed for antibiotic monitoring in a food product. The modification of SPCE with PEDOT/GO as a sensor platform was performed through electropolymerization, while the electrochemical assay was accomplished while using direct competitive format in which the free CLB and clenbuterol-horseradish peroxidase (CLB-HRP) in the solution will compete to form binding with the polyclonal anti-clenbuterol antibody (Ab) immobilized onto the modified electrode surface. A linear standard CLB calibration curve with R2 = 0.9619 and low limit of detection (0.196 ng mL−1) was reported. Analysis of milk samples indicated that this immunosensor was able to detect CLB in real samples and the results that were obtained were comparable with enzyme-linked immunosorbent assays (ELISA). View Full-Text
Keywords: clenbuterol; poly(3,4-ethylenedioxythiophene); graphene oxide; immunosensor; electrochemical clenbuterol; poly(3,4-ethylenedioxythiophene); graphene oxide; immunosensor; electrochemical
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Talib, N.A.A.; Salam, F.; Sulaiman, Y. Development of Highly Sensitive Immunosensor for Clenbuterol Detection by Using Poly(3,4-ethylenedioxythiophene)/Graphene Oxide Modified Screen-Printed Carbon Electrode. Sensors 2018, 18, 4324.

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